Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4746868..4747678 Replicon chromosome
Accession NZ_CP100110
Organism Klebsiella pneumoniae strain HSKP104

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NIL12_RS23085 Protein ID WP_004178461.1
Coordinates 4746868..4747401 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NIL12_RS23090 Protein ID WP_002887278.1
Coordinates 4747412..4747678 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL12_RS23080 (4745699) 4745699..4746820 + 1122 WP_002887282.1 cupin domain-containing protein -
NIL12_RS23085 (4746868) 4746868..4747401 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NIL12_RS23090 (4747412) 4747412..4747678 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NIL12_RS23095 (4747781) 4747781..4749214 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
NIL12_RS23100 (4749204) 4749204..4749887 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NIL12_RS23105 (4750059) 4750059..4751444 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
NIL12_RS23110 (4751462) 4751462..4751806 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T250197 WP_004178461.1 NZ_CP100110:c4747401-4746868 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250197 WP_002887278.1 NZ_CP100110:c4747678-4747412 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References