Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 14475..15220 Replicon plasmid pHSKP33-1
Accession NZ_CP100103
Organism Klebsiella pneumoniae strain HSKP33

Toxin (Protein)


Gene name tacT Uniprot ID A0A331KSM6
Locus tag NIL14_RS26150 Protein ID WP_032408901.1
Coordinates 14729..15220 (+) Length 164 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag NIL14_RS26145 Protein ID WP_014386183.1
Coordinates 14475..14741 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL14_RS26095 (NIL14_26080) 10029..10442 - 414 WP_013023817.1 helix-turn-helix domain-containing protein -
NIL14_RS26100 (NIL14_26085) 10443..10721 - 279 WP_004152721.1 helix-turn-helix transcriptional regulator -
NIL14_RS26105 (NIL14_26090) 10711..11031 - 321 WP_014386190.1 type II toxin-antitoxin system RelE/ParE family toxin -
NIL14_RS26110 (NIL14_26095) 11112..11336 - 225 WP_014386189.1 hypothetical protein -
NIL14_RS26115 (NIL14_26100) 11347..11559 - 213 WP_014386188.1 hypothetical protein -
NIL14_RS26120 (NIL14_26105) 11621..11947 - 327 WP_014386187.1 hypothetical protein -
NIL14_RS26125 (NIL14_26110) 12582..12932 - 351 WP_014386186.1 hypothetical protein -
NIL14_RS26130 (NIL14_26115) 12929..13201 - 273 WP_032408902.1 hypothetical protein -
NIL14_RS26135 (NIL14_26120) 13391..13876 + 486 WP_014386185.1 hypothetical protein -
NIL14_RS26140 (NIL14_26125) 14120..14278 - 159 WP_014386184.1 type I toxin-antitoxin system Hok family toxin -
NIL14_RS26145 (NIL14_26130) 14475..14741 + 267 WP_014386183.1 DUF1778 domain-containing protein Antitoxin
NIL14_RS26150 (NIL14_26135) 14729..15220 + 492 WP_032408901.1 GNAT family N-acetyltransferase Toxin
NIL14_RS26155 (NIL14_26140) 15661..15912 - 252 WP_032408900.1 aldo/keto reductase -
NIL14_RS26160 (NIL14_26145) 16109..17701 - 1593 Protein_28 IS66 family transposase -
NIL14_RS26165 (NIL14_26150) 17732..18082 - 351 WP_014386180.1 IS66 family insertion sequence element accessory protein TnpB -
NIL14_RS26170 (NIL14_26155) 18079..18519 - 441 WP_014386179.1 transposase -
NIL14_RS26175 (NIL14_26160) 18781..19536 - 756 WP_032408898.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..175328 175328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(89-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17667.46 Da        Isoelectric Point: 8.8757

>T250163 WP_032408901.1 NZ_CP100103:14729-15220 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Download         Length: 492 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.66 Da        Isoelectric Point: 5.1169

>AT250163 WP_014386183.1 NZ_CP100103:14475-14741 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331KSM6

Antitoxin

Source ID Structure

References