Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2842253..2842878 Replicon chromosome
Accession NZ_CP100102
Organism Klebsiella pneumoniae strain HSKP33

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NIL14_RS13625 Protein ID WP_002882817.1
Coordinates 2842253..2842636 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NIL14_RS13630 Protein ID WP_004150355.1
Coordinates 2842636..2842878 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL14_RS13610 (2839619) 2839619..2840521 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NIL14_RS13615 (2840518) 2840518..2841153 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NIL14_RS13620 (2841150) 2841150..2842079 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NIL14_RS13625 (2842253) 2842253..2842636 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NIL14_RS13630 (2842636) 2842636..2842878 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NIL14_RS13635 (2843083) 2843083..2844000 + 918 WP_004181612.1 alpha/beta hydrolase -
NIL14_RS13640 (2844014) 2844014..2844955 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NIL14_RS13645 (2845000) 2845000..2845437 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NIL14_RS13650 (2845434) 2845434..2846294 - 861 WP_004146232.1 virulence factor BrkB family protein -
NIL14_RS13655 (2846288) 2846288..2846887 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250155 WP_002882817.1 NZ_CP100102:c2842636-2842253 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250155 WP_004150355.1 NZ_CP100102:c2842878-2842636 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References