Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5326114..5326739 Replicon chromosome
Accession NZ_CP100098
Organism Klebsiella pneumoniae strain HSKP43

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NIL15_RS25760 Protein ID WP_002882817.1
Coordinates 5326114..5326497 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NIL15_RS25765 Protein ID WP_004150355.1
Coordinates 5326497..5326739 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL15_RS25745 (5323480) 5323480..5324382 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NIL15_RS25750 (5324379) 5324379..5325014 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NIL15_RS25755 (5325011) 5325011..5325940 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NIL15_RS25760 (5326114) 5326114..5326497 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NIL15_RS25765 (5326497) 5326497..5326739 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NIL15_RS25770 (5326944) 5326944..5327861 + 918 WP_004181612.1 alpha/beta hydrolase -
NIL15_RS25775 (5327875) 5327875..5328816 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NIL15_RS25780 (5328861) 5328861..5329298 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NIL15_RS25785 (5329295) 5329295..5330155 - 861 WP_004146232.1 virulence factor BrkB family protein -
NIL15_RS25790 (5330149) 5330149..5330748 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250143 WP_002882817.1 NZ_CP100098:c5326497-5326114 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250143 WP_004150355.1 NZ_CP100098:c5326739-5326497 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References