Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4746861..4747671 Replicon chromosome
Accession NZ_CP100098
Organism Klebsiella pneumoniae strain HSKP43

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NIL15_RS23085 Protein ID WP_004178461.1
Coordinates 4746861..4747394 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NIL15_RS23090 Protein ID WP_002887278.1
Coordinates 4747405..4747671 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL15_RS23080 (4745692) 4745692..4746813 + 1122 WP_002887282.1 cupin domain-containing protein -
NIL15_RS23085 (4746861) 4746861..4747394 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NIL15_RS23090 (4747405) 4747405..4747671 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NIL15_RS23095 (4747774) 4747774..4749207 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
NIL15_RS23100 (4749197) 4749197..4749880 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NIL15_RS23105 (4750052) 4750052..4751437 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
NIL15_RS23110 (4751455) 4751455..4751799 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T250140 WP_004178461.1 NZ_CP100098:c4747394-4746861 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250140 WP_002887278.1 NZ_CP100098:c4747671-4747405 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References