Detailed information of TA system TA025013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3872217..3872758
• Replicon: chromosome
• Accession: NC_014830
• Organism: Intrasporangium calvum DSM 43043

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: INTCA_RS19300
• Protein ID: WP_013494289.1
• Coordinates: 3872217..3872495 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E6SG24
• Locus tag: INTCA_RS17645
• Protein ID: WP_013494290.1
• Coordinates: 3872492..3872758 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INTCA_RS17615	3867301..3868443	+	1143	WP_013494283.1	zinc-binding dehydrogenase	-
INTCA_RS17620	3868720..3869058	-	339	WP_013494284.1	type II toxin-antitoxin system PemK/MazF family toxin	-
INTCA_RS17625	3869042..3869239	-	198	WP_013494285.1	hypothetical protein	-
INTCA_RS17630	3869431..3869919	+	489	WP_148236665.1	hypothetical protein	-
INTCA_RS17635	3870250..3871545	+	1296	WP_013494287.1	IS110 family transposase	-
INTCA_RS18825	3871615..3872079	-	465	WP_052338053.1	cupin domain-containing protein	-
INTCA_RS19300	3872217..3872495	-	279	WP_013494289.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INTCA_RS17645	3872492..3872758	-	267	WP_013494290.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
INTCA_RS17650	3872929..3873615	-	687	WP_148236667.1	tyrosine-type recombinase/integrase	-
INTCA_RS17655	3873670..3875007	-	1338	Protein_3487	hypothetical protein	-
INTCA_RS17670	3876794..3877117	-	324	WP_013491643.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3861137..3883088	21951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (31-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.80 Da
• Isoelectric Point: 10.3818

>T25013 WP_013494289.1 NC_014830:c3872495-3872217 [Intrasporangium calvum DSM 43043]
VSEEPYELVLTPPAVRAVRSGLPEAVAAAVIEFLTGALIQNPHRVGKPLRGELAGIHSARRGTYRVLYRINEIQGEVVVL
RIDHRRDAYRRG

Nucleotide

• Length: 279 bp

>T25013 NC_014830:c3872495-3872217 [Intrasporangium calvum DSM 43043]
GTGAGCGAAGAACCGTACGAACTGGTCCTGACCCCTCCCGCCGTCCGAGCCGTGCGCTCAGGACTGCCCGAGGCCGTTGC
GGCGGCGGTCATAGAGTTCCTCACCGGAGCATTGATCCAGAACCCGCACCGCGTCGGCAAGCCATTGCGGGGGGAGCTCG
CGGGTATCCACTCGGCCCGGCGCGGCACCTACCGCGTCCTGTATCGAATCAACGAGATCCAAGGCGAAGTGGTGGTTCTC
CGCATCGACCACCGACGAGACGCTTACCGACGCGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9717.81 Da
• Isoelectric Point: 4.4325

>AT25013 WP_013494290.1 NC_014830:c3872758-3872492 [Intrasporangium calvum DSM 43043]
MTVEPLRDVRNHFSDVIDRVEHEHERVTVTRNGRPVAVILSPDDLAQLEETLSVLSDPEALADIREADAAYATGDVVRGV
EAVRGLRS

Nucleotide

• Length: 267 bp

>AT25013 NC_014830:c3872758-3872492 [Intrasporangium calvum DSM 43043]
ATGACTGTTGAACCGCTTCGCGATGTCCGCAACCATTTCAGCGACGTCATCGACCGCGTCGAGCATGAGCACGAGCGGGT
CACAGTCACGCGCAATGGACGCCCGGTTGCCGTCATCCTCAGCCCGGACGACCTGGCGCAGCTGGAGGAGACCCTCTCGG
TGCTCAGCGACCCCGAAGCCCTCGCCGACATCCGTGAGGCCGATGCGGCATACGCGACCGGTGACGTCGTTCGCGGGGTC
GAGGCCGTGCGCGGCCTGCGCTCGTGA

Structures

Antitoxin

• AlphaFold DB: E6SG24