Detailed information of TA system TA025010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 2327025..2327578
• Replicon: chromosome
• Accession: NC_014830
• Organism: Intrasporangium calvum DSM 43043

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: INTCA_RS10535
• Protein ID: WP_052338114.1
• Coordinates: 2327025..2327249 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E6SCZ8
• Locus tag: INTCA_RS10540
• Protein ID: WP_013492901.1
• Coordinates: 2327297..2327578 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INTCA_RS10505	2322645..2322986	-	342	WP_013492896.1	bifunctional 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit	-
INTCA_RS10510	2323071..2323730	-	660	WP_013492897.1	metalloregulator ArsR/SmtB family transcription factor	-
INTCA_RS10515	2323727..2325037	-	1311	WP_041308631.1	MFS transporter	-
INTCA_RS10525	2325759..2326079	-	321	WP_041307546.1	IS110 family transposase	-
INTCA_RS10530	2326599..2326910	+	312	WP_041307550.1	hypothetical protein	-
INTCA_RS10535	2327025..2327249	-	225	WP_052338114.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INTCA_RS10540	2327297..2327578	-	282	WP_013492901.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
INTCA_RS10545	2328009..2328482	+	474	WP_013492902.1	hypothetical protein	-
INTCA_RS10550	2328492..2328845	+	354	WP_013492903.1	hypothetical protein	-
INTCA_RS10555	2329100..2329426	-	327	WP_169312955.1	metalloregulator ArsR/SmtB family transcription factor	-
INTCA_RS10560	2329590..2330720	+	1131	WP_013492906.1	ACR3 family arsenite efflux transporter	-
INTCA_RS10565	2330713..2331159	+	447	WP_013492907.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (20-72)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8371.53 Da
• Isoelectric Point: 8.5956

>T25010 WP_052338114.1 NC_014830:c2327249-2327025 [Intrasporangium calvum DSM 43043]
MTEDLPEAVATAAFEFITGPLLENPHRVGKQLRPPLTDRHSARRGTYRILYRIDEQAQTVTVLAIGHRGDIYRA

Nucleotide

• Length: 225 bp

>T25010 NC_014830:c2327249-2327025 [Intrasporangium calvum DSM 43043]
ATGACTGAAGATCTCCCAGAAGCCGTCGCCACCGCCGCCTTCGAGTTCATCACCGGCCCCTTGCTCGAGAACCCTCACCG
CGTGGGCAAGCAACTGCGCCCACCCCTGACAGACCGACACAGCGCACGACGTGGCACCTATCGCATCCTCTACCGCATCG
ACGAACAAGCCCAGACGGTGACCGTCCTAGCGATCGGCCACCGTGGCGACATCTACCGTGCGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 9902.22 Da
• Isoelectric Point: 5.0600

>AT25010 WP_013492901.1 NC_014830:c2327578-2327297 [Intrasporangium calvum DSM 43043]
MTSIPLADAKAHLSEVISRVSKHHERVTVTVHGQPSAVLLAPDDLAMLEETIAVLGDRDLMTQLATADADLAAGRVETQG
DLEAAMRSRRTSA

Nucleotide

• Length: 282 bp

>AT25010 NC_014830:c2327578-2327297 [Intrasporangium calvum DSM 43043]
ATGACGAGCATTCCACTGGCCGACGCCAAGGCACACCTGTCCGAGGTCATCAGCCGAGTGAGCAAGCACCACGAACGAGT
AACCGTGACCGTGCACGGTCAGCCCTCGGCCGTCCTGCTGGCGCCCGATGACCTCGCGATGCTCGAGGAGACCATCGCCG
TCTTGGGGGACCGCGACCTCATGACGCAGCTTGCCACCGCCGACGCCGACCTGGCTGCCGGCCGTGTCGAGACCCAAGGC
GACCTCGAGGCGGCGATGCGGTCACGCCGGACCTCGGCGTGA

Structures

Antitoxin

• AlphaFold DB: E6SCZ8