Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 498383..499008 Replicon chromosome
Accession NZ_CP100087
Organism Klebsiella pneumoniae strain HSKP8

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NIL17_RS02370 Protein ID WP_002882817.1
Coordinates 498383..498766 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NIL17_RS02375 Protein ID WP_004150355.1
Coordinates 498766..499008 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL17_RS02355 (495749) 495749..496651 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NIL17_RS02360 (496648) 496648..497283 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NIL17_RS02365 (497280) 497280..498209 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NIL17_RS02370 (498383) 498383..498766 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NIL17_RS02375 (498766) 498766..499008 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NIL17_RS02380 (499213) 499213..500130 + 918 WP_004181612.1 alpha/beta hydrolase -
NIL17_RS02385 (500144) 500144..501085 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NIL17_RS02390 (501130) 501130..501567 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NIL17_RS02395 (501564) 501564..502424 - 861 WP_004146232.1 virulence factor BrkB family protein -
NIL17_RS02400 (502418) 502418..503017 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250093 WP_002882817.1 NZ_CP100087:c498766-498383 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250093 WP_004150355.1 NZ_CP100087:c499008-498766 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References