Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4782137..4782947 Replicon chromosome
Accession NZ_CP100084
Organism Klebsiella pneumoniae strain HSKP86

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NIL18_RS23255 Protein ID WP_004178461.1
Coordinates 4782137..4782670 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NIL18_RS23260 Protein ID WP_002887278.1
Coordinates 4782681..4782947 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL18_RS23250 (4780968) 4780968..4782089 + 1122 WP_002887282.1 cupin domain-containing protein -
NIL18_RS23255 (4782137) 4782137..4782670 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NIL18_RS23260 (4782681) 4782681..4782947 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NIL18_RS23265 (4783050) 4783050..4784483 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
NIL18_RS23270 (4784473) 4784473..4785156 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NIL18_RS23275 (4785328) 4785328..4786713 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
NIL18_RS23280 (4786731) 4786731..4787075 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T250082 WP_004178461.1 NZ_CP100084:c4782670-4782137 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250082 WP_002887278.1 NZ_CP100084:c4782947-4782681 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References