Detailed information of TA system TA025008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 363882..364512
• Replicon: chromosome
• Accession: NC_014829
• Organism: Bacillus cellulosilyticus DSM 2522

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: BCELL_RS01805
• Protein ID: WP_041808099.1
• Coordinates: 364162..364512 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: E6TV43
• Locus tag: BCELL_RS01800
• Protein ID: WP_013486967.1
• Coordinates: 363882..364157 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCELL_RS01780	359312..359671	+	360	WP_013486963.1	holo-ACP synthase	-
BCELL_RS01785	359748..361280	+	1533	WP_013486964.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
BCELL_RS01790	361328..362374	+	1047	WP_013486965.1	outer membrane lipoprotein carrier protein LolA	-
BCELL_RS01795	362569..363714	+	1146	WP_013486966.1	alanine racemase	-
BCELL_RS01800	363882..364157	+	276	WP_013486967.1	hypothetical protein	Antitoxin
BCELL_RS01805	364162..364512	+	351	WP_041808099.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCELL_RS01810	364830..365654	+	825	WP_013486969.1	STAS domain-containing protein	-
BCELL_RS01815	365666..366022	+	357	WP_013486970.1	STAS domain-containing protein	-
BCELL_RS01820	366025..366429	+	405	WP_013486971.1	anti-sigma regulatory factor	-
BCELL_RS01825	366465..367457	+	993	WP_157184246.1	PP2C family protein-serine/threonine phosphatase	-
BCELL_RS01830	367513..367842	+	330	WP_013486973.1	anti-sigma factor antagonist	-
BCELL_RS01835	367845..368336	+	492	WP_013486974.1	anti-sigma B factor RsbW	-
BCELL_RS01840	368299..369087	+	789	WP_013486975.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12946.07 Da
• Isoelectric Point: 7.2025

>T25008 WP_041808099.1 NC_014829:364162-364512 [Bacillus cellulosilyticus DSM 2522]
MIVKRGDVYFADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFDRDSVILLEQV
RTIDKQRLTDKITHLDDDMMVKVNDALRISLGLIDF

Nucleotide

• Length: 351 bp

>T25008 NC_014829:364162-364512 [Bacillus cellulosilyticus DSM 2522]
TTGATTGTAAAACGTGGGGACGTTTATTTTGCAGACCTTTCACCCGTGGTTGGATCTGAGCAAGGTGGGATTCGACCAGT
ATTGATTATCCAAAATGATATTGGAAATCGTTTTAGCCCAACGGTCATTGTCGCAGCAATTACAGCACAGATTCAAAAAG
CTAAATTGCCGACACACGTTGAAATCAATGCGAAAAAATACGGGTTTGATAGAGACTCCGTTATTTTATTAGAACAGGTT
CGCACGATTGATAAGCAACGATTAACTGACAAAATCACTCATCTAGACGATGACATGATGGTAAAAGTGAATGATGCCTT
ACGTATAAGTTTAGGTTTGATTGACTTTTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10384.94 Da
• Isoelectric Point: 4.9155

>AT25008 WP_013486967.1 NC_014829:363882-364157 [Bacillus cellulosilyticus DSM 2522]
VSEESTKKIMVSLPQELVNELDGLLEGNVNRSELIHKATKMYLKEHKKQIIRETMQQGYMEMAKINLNIATEAFLAEEEA
EHTLDRLVSGV

Nucleotide

• Length: 276 bp

>AT25008 NC_014829:363882-364157 [Bacillus cellulosilyticus DSM 2522]
GTGTCTGAAGAGAGCACAAAAAAAATAATGGTGAGCTTACCACAAGAGCTCGTTAATGAATTAGATGGTTTATTAGAGGG
TAATGTTAACCGTAGCGAATTAATACACAAAGCTACGAAAATGTATCTAAAGGAACATAAAAAACAAATAATTCGTGAAA
CGATGCAACAAGGCTATATGGAAATGGCAAAAATTAATTTAAACATCGCGACAGAAGCCTTTCTTGCTGAGGAGGAAGCC
GAGCACACGTTGGATCGCTTAGTAAGTGGGGTGTAA

Structures

Antitoxin

• AlphaFold DB: E6TV43