Detailed information of TA system TA025007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 557523..558076
• Replicon: chromosome
• Accession: NC_014828
• Organism: Ethanoligenens harbinense YUAN-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ETHHA_RS02755
• Protein ID: WP_083803592.1
• Coordinates: 557523..557750 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E6U9C3
• Locus tag: ETHHA_RS02760
• Protein ID: WP_013484486.1
• Coordinates: 557801..558076 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETHHA_RS02730	553176..554327	+	1152	WP_013484482.1	ABC transporter permease	-
ETHHA_RS15760	554440..554805	-	366	WP_175405609.1	hypothetical protein	-
ETHHA_RS02745	555163..555594	+	432	WP_013484484.1	sigma-70 family RNA polymerase sigma factor	-
ETHHA_RS14925	555649..555843	+	195	WP_083803591.1	DUF3847 domain-containing protein	-
ETHHA_RS02750	556038..557474	+	1437	Protein_529	MobA/MobL family protein	-
ETHHA_RS02755	557523..557750	-	228	WP_083803592.1	type II toxin-antitoxin system YafQ family toxin	Toxin
ETHHA_RS02760	557801..558076	-	276	WP_013484486.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ETHHA_RS02765	558541..558741	+	201	WP_013484488.1	transposon-encoded TnpW family protein	-
ETHHA_RS14930	559004..559114	+	111	Protein_533	transposase	-
ETHHA_RS02770	559348..560679	+	1332	WP_013484489.1	ferredoxin reductase family protein	-
ETHHA_RS14935	560714..561097	+	384	WP_013484490.1	cytochrome b5	-
ETHHA_RS02780	561180..561431	+	252	WP_041687065.1	SHOCT domain-containing protein	-
ETHHA_RS02785	561488..562174	+	687	WP_041686605.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	542840..641591	98751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-73)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-87)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8692.04 Da
• Isoelectric Point: 8.0648

>T25007 WP_083803592.1 NC_014828:c557750-557523 [Ethanoligenens harbinense YUAN-3]
MKRGLKMDLLDNIIRQLSKGQLLPEKNRDHLLTGNWVGYRECHIQSDWLLVYRIENGVLVLTLSRTGSHSDLFGE

Nucleotide

• Length: 228 bp

>T25007 NC_014828:c557750-557523 [Ethanoligenens harbinense YUAN-3]
ATGAAGCGCGGGCTGAAAATGGATTTGCTGGACAATATCATCCGTCAGCTTTCCAAAGGTCAGCTTTTACCAGAGAAGAA
CCGCGATCATCTTCTGACCGGAAATTGGGTGGGATACCGGGAATGCCATATTCAAAGCGATTGGCTGCTTGTGTATCGAA
TTGAGAATGGCGTTCTGGTTTTAACGCTGTCCCGTACAGGCTCGCACAGTGATTTGTTTGGAGAGTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10138.54 Da
• Isoelectric Point: 6.2990

>AT25007 WP_013484486.1 NC_014828:c558076-557801 [Ethanoligenens harbinense YUAN-3]
MANTTNFSVRMDSDIKKQCEALYSELGVNLTTAINVFLRQSLRSGGFPFDVTLNTPNAVTLAAMQEAERLAKDPNAKRFS
DVEEALRELKK

Nucleotide

• Length: 276 bp

>AT25007 NC_014828:c558076-557801 [Ethanoligenens harbinense YUAN-3]
ATGGCCAATACGACAAATTTCAGCGTCCGCATGGACAGCGATATTAAAAAACAGTGCGAGGCGCTTTACAGTGAACTCGG
TGTGAATCTCACGACGGCTATCAACGTGTTTTTGCGGCAATCGCTTCGATCTGGCGGTTTCCCGTTCGACGTGACGCTCA
ACACGCCGAACGCCGTTACCCTCGCCGCCATGCAGGAAGCGGAGCGGCTTGCTAAAGACCCCAACGCAAAACGCTTCAGC
GATGTGGAGGAAGCGTTGAGGGAACTGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A386XKC6