Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4588088..4588898 Replicon chromosome
Accession NZ_CP100076
Organism Klebsiella pneumoniae strain RJKP36

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NIL00_RS22150 Protein ID WP_004178461.1
Coordinates 4588088..4588621 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NIL00_RS22155 Protein ID WP_002887278.1
Coordinates 4588632..4588898 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL00_RS22145 (4586919) 4586919..4588040 + 1122 WP_002887282.1 cupin domain-containing protein -
NIL00_RS22150 (4588088) 4588088..4588621 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NIL00_RS22155 (4588632) 4588632..4588898 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NIL00_RS22160 (4589001) 4589001..4590434 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
NIL00_RS22165 (4590424) 4590424..4591107 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NIL00_RS22170 (4591279) 4591279..4592664 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
NIL00_RS22175 (4592682) 4592682..4593026 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T250064 WP_004178461.1 NZ_CP100076:c4588621-4588088 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250064 WP_002887278.1 NZ_CP100076:c4588898-4588632 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References