Detailed information of TA system TA025006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 191974..192614
• Replicon: plasmid pASTEX01
• Accession: NC_014818
• Organism: Asticcacaulis excentricus CB 48

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ASTEX_RS20025
• Protein ID: WP_013481091.1
• Coordinates: 191974..192393 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ASTEX_RS18155
• Protein ID: WP_013481092.1
• Coordinates: 192393..192614 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASTEX_RS18120	186992..188083	+	1092	WP_013481083.1	putative urea ABC transporter substrate-binding protein	-
ASTEX_RS18125	188092..188910	+	819	WP_013481084.1	ABC transporter permease subunit	-
ASTEX_RS18130	188907..189686	+	780	WP_013481085.1	ATP-binding cassette domain-containing protein	-
ASTEX_RS18135	190005..190385	-	381	WP_013481087.1	type II toxin-antitoxin system death-on-curing family toxin	-
ASTEX_RS18140	190385..190618	-	234	WP_013481088.1	hypothetical protein	-
ASTEX_RS18150	191538..191816	-	279	WP_013481090.1	DUF1778 domain-containing protein	-
ASTEX_RS20025	191974..192393	-	420	WP_013481091.1	type II toxin-antitoxin system VapC family toxin	Toxin
ASTEX_RS18155	192393..192614	-	222	WP_013481092.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ASTEX_RS18160	193233..193532	-	300	WP_013481093.1	hypothetical protein	-
ASTEX_RS18165	193725..194060	+	336	WP_041659726.1	helix-turn-helix domain-containing protein	-
ASTEX_RS18170	194053..195300	+	1248	WP_013481095.1	HipA domain-containing protein	-
ASTEX_RS18175	195536..196168	-	633	WP_041659727.1	TetR/AcrR family transcriptional regulator	-
ASTEX_RS18180	196312..197349	+	1038	WP_049781783.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..244260	244260

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (9-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15402.52 Da
• Isoelectric Point: 6.7157

>T25006 WP_013481091.1 NC_014818:c192393-191974 [Asticcacaulis excentricus CB 48]
MLRYMLDTNLCIRVLRDRPQSIRERFNAEADGLCISTIVLAEFLHGAEKSDRPAFNRTKVEQFAARLEVIAFGSEAAAHA
ADIRASLERRGTPIGVYDVLIAGHARSRGLVVVTGNLSEFSRVEGLRSEDWLSSDPMAN

Nucleotide

• Length: 420 bp

>T25006 NC_014818:c192393-191974 [Asticcacaulis excentricus CB 48]
ATGCTTCGATATATGCTTGATACAAATCTATGCATCAGAGTGCTCCGGGACCGGCCGCAGTCTATCCGCGAACGGTTCAA
CGCTGAAGCTGATGGGCTTTGTATCTCAACTATAGTATTGGCGGAATTTTTACACGGTGCTGAAAAGTCTGACCGCCCGG
CTTTTAACCGAACAAAAGTCGAGCAGTTTGCTGCGCGGCTGGAGGTAATTGCATTTGGGTCAGAGGCTGCCGCCCACGCA
GCAGACATTCGAGCGTCTTTGGAACGTAGGGGTACTCCGATTGGCGTTTATGATGTACTAATTGCAGGTCATGCCCGAAG
CAGAGGCTTGGTTGTTGTCACTGGAAATTTGAGTGAGTTCTCCCGCGTTGAAGGGCTGCGGAGCGAGGACTGGCTTTCGT
CCGACCCGATGGCAAACTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8297.41 Da
• Isoelectric Point: 7.0127

>AT25006 WP_013481092.1 NC_014818:c192614-192393 [Asticcacaulis excentricus CB 48]
MARTTLFHSNRSQAVRLPKDVAFPEGVKDVTILREGGRRIIVPSNSIWDDFFAAPGIDLGERDQLAEQVRERF

Nucleotide

• Length: 222 bp

>AT25006 NC_014818:c192614-192393 [Asticcacaulis excentricus CB 48]
ATGGCCCGTACGACCTTGTTTCATTCGAATCGCTCGCAAGCAGTTCGCTTACCTAAGGATGTGGCTTTTCCGGAAGGCGT
CAAAGACGTGACTATTTTGAGGGAAGGGGGGCGTCGTATCATCGTGCCGTCTAACAGCATTTGGGACGATTTTTTTGCGG
CCCCAGGCATTGATCTTGGCGAGCGCGACCAACTGGCCGAACAAGTCCGTGAGCGCTTCTAA