Detailed information of TA system TA025005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 693664..694294
• Replicon: chromosome
• Accession: NC_014817
• Organism: Asticcacaulis excentricus CB 48

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E8RT17
• Locus tag: ASTEX_RS14910
• Protein ID: WP_013480462.1
• Coordinates: 693884..694294 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E8RT16
• Locus tag: ASTEX_RS14905
• Protein ID: WP_013480461.1
• Coordinates: 693664..693894 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASTEX_RS14885	689727..691130	+	1404	WP_013480457.1	M48 family metallopeptidase	-
ASTEX_RS14890	691157..691957	+	801	WP_013480458.1	thioredoxin domain-containing protein	-
ASTEX_RS14895	692070..692699	+	630	WP_013480459.1	hypothetical protein	-
ASTEX_RS14900	692708..693502	-	795	WP_013480460.1	thiazole synthase	-
ASTEX_RS14905	693664..693894	+	231	WP_013480461.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ASTEX_RS14910	693884..694294	+	411	WP_013480462.1	PIN domain-containing protein	Toxin
ASTEX_RS14915	694415..694900	-	486	WP_013480463.1	hypothetical protein	-
ASTEX_RS14920	694902..695105	-	204	WP_013480464.1	sulfur carrier protein ThiS	-
ASTEX_RS14925	695199..695639	+	441	WP_013480465.1	type II 3-dehydroquinate dehydratase	-
ASTEX_RS14930	695691..696182	+	492	WP_013480466.1	acetyl-CoA carboxylase biotin carboxyl carrier protein	-
ASTEX_RS14935	696205..697566	+	1362	WP_041659580.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
ASTEX_RS14940	697982..698635	+	654	WP_144004750.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-116)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15270.55 Da
• Isoelectric Point: 4.7657

>T25005 WP_013480462.1 NC_014817:693884-694294 [Asticcacaulis excentricus CB 48]
MAAERVFFDTNVLVYLLKDDRHKGECCADLIAGGGTISVQVLNELLNVIRRKTDLNWPDTLRFLETLEGLFEITDMTQET
HRLGRRLAERYRLSVYDALIIAASILSGASVLYTEDMPDGAIIEGVRIVNPFTGRP

Nucleotide

• Length: 411 bp

>T25005 NC_014817:693884-694294 [Asticcacaulis excentricus CB 48]
ATGGCCGCTGAGCGCGTCTTCTTCGACACCAATGTGCTGGTCTATTTGCTTAAGGACGACAGGCACAAGGGCGAGTGCTG
CGCCGACCTCATCGCTGGCGGAGGTACGATCAGCGTGCAGGTGCTGAACGAACTGCTGAATGTCATCCGCCGCAAGACCG
ACCTGAACTGGCCTGACACCCTGCGCTTTCTGGAAACGCTGGAAGGGCTGTTCGAGATTACCGACATGACGCAGGAGACC
CACCGACTGGGGCGGCGTCTGGCGGAACGCTATCGTCTGTCGGTCTATGATGCCCTGATTATTGCCGCCTCTATCCTCTC
CGGGGCCAGCGTCCTTTATACTGAAGATATGCCAGACGGTGCCATCATCGAAGGCGTGCGCATCGTCAACCCATTCACGG
GCAGGCCTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8516.69 Da
• Isoelectric Point: 7.0136

>AT25005 WP_013480461.1 NC_014817:693664-693894 [Asticcacaulis excentricus CB 48]
MQVSKWGNSLAVRLPAAVVEALELKDGDEIEIHVQDARQFAIARKPDRDALIARLRGFAGRLPADFKFDRDDVNGR

Nucleotide

• Length: 231 bp

>AT25005 NC_014817:693664-693894 [Asticcacaulis excentricus CB 48]
ATGCAGGTTTCCAAATGGGGCAACAGTCTGGCGGTGCGCCTGCCCGCCGCCGTGGTCGAAGCGCTGGAACTGAAGGACGG
CGACGAAATCGAAATCCACGTGCAGGACGCGCGGCAATTCGCTATTGCGCGCAAGCCTGACCGGGACGCCCTGATCGCGC
GCCTGCGCGGCTTTGCGGGTCGTCTGCCAGCCGATTTCAAATTTGACCGCGACGACGTAAATGGCCGCTGA

Structures

Toxin

• AlphaFold DB: E8RT17

Antitoxin

• AlphaFold DB: E8RT16