Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4737445..4738255 Replicon chromosome
Accession NZ_CP100072
Organism Klebsiella pneumoniae strain RJKP41

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NIL01_RS23035 Protein ID WP_004178461.1
Coordinates 4737445..4737978 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NIL01_RS23040 Protein ID WP_002887278.1
Coordinates 4737989..4738255 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL01_RS23030 (4736276) 4736276..4737397 + 1122 WP_002887282.1 cupin domain-containing protein -
NIL01_RS23035 (4737445) 4737445..4737978 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NIL01_RS23040 (4737989) 4737989..4738255 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NIL01_RS23045 (4738358) 4738358..4739791 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
NIL01_RS23050 (4739781) 4739781..4740464 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NIL01_RS23055 (4740636) 4740636..4742021 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
NIL01_RS23060 (4742039) 4742039..4742383 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T250041 WP_004178461.1 NZ_CP100072:c4737978-4737445 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250041 WP_002887278.1 NZ_CP100072:c4738255-4737989 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References