Detailed information of TA system TA025004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 1871496..1872082
• Replicon: chromosome
• Accession: NC_014816
• Organism: Asticcacaulis excentricus CB 48

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E8RRF7
• Locus tag: ASTEX_RS08625
• Protein ID: WP_013479232.1
• Coordinates: 1871496..1871771 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E8RRF8
• Locus tag: ASTEX_RS08630
• Protein ID: WP_013479233.1
• Coordinates: 1871768..1872082 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASTEX_RS08605	1867290..1868096	+	807	WP_013479228.1	thymidylate synthase	-
ASTEX_RS08610	1868255..1868776	+	522	WP_013479229.1	dihydrofolate reductase	-
ASTEX_RS08615	1869026..1870117	-	1092	WP_013479230.1	NADH:flavin oxidoreductase	-
ASTEX_RS08620	1870268..1871473	+	1206	WP_013479231.1	DUF1624 domain-containing protein	-
ASTEX_RS08625	1871496..1871771	-	276	WP_013479232.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASTEX_RS08630	1871768..1872082	-	315	WP_013479233.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ASTEX_RS08635	1872131..1873237	-	1107	WP_013479234.1	Mrp/NBP35 family ATP-binding protein	-
ASTEX_RS08640	1873315..1874169	+	855	WP_013479235.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
ASTEX_RS08645	1874232..1876178	+	1947	WP_013479236.1	S9 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-53)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10102.38 Da
• Isoelectric Point: 5.0069

>T25004 WP_013479232.1 NC_014816:c1871771-1871496 [Asticcacaulis excentricus CB 48]
MIIQWSASALDDRIKTFQFIADDSVTAAARVNEAVDAAAEHLADFPDSGRPGRAPSTREWVLNGLPYLLIESVEGHSLTI
WRVLHTSRLWA

Nucleotide

• Length: 276 bp

>T25004 NC_014816:c1871771-1871496 [Asticcacaulis excentricus CB 48]
ATGATCATTCAGTGGTCGGCATCGGCGCTTGATGACCGTATTAAAACGTTTCAGTTTATTGCCGATGACAGTGTGACCGC
GGCCGCGAGGGTGAATGAGGCGGTAGACGCCGCGGCGGAACATCTGGCGGATTTTCCGGACAGTGGCAGACCGGGACGTG
CTCCGAGTACACGCGAATGGGTGCTGAACGGGCTGCCTTATCTGCTGATTGAAAGCGTTGAAGGCCACAGTCTCACCATC
TGGCGCGTCCTGCATACCTCACGGCTGTGGGCGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11419.96 Da
• Isoelectric Point: 9.9862

>AT25004 WP_013479233.1 NC_014816:c1872082-1871768 [Asticcacaulis excentricus CB 48]
VRWRASEVVQARIDGVIKERAGAVLSQIGLTVSDVVRSLLTRFTNEGALPAGLTGDSQAYDVWLRDKVREAMEDQRPPVS
HADAHARMADIKAQVLARRDAKGQ

Nucleotide

• Length: 315 bp

>AT25004 NC_014816:c1872082-1871768 [Asticcacaulis excentricus CB 48]
GTGCGATGGCGGGCAAGTGAAGTGGTGCAGGCGCGCATTGACGGCGTGATCAAGGAGCGGGCCGGGGCTGTGCTCAGCCA
GATTGGGCTGACGGTGTCCGACGTCGTTCGTAGTCTGTTGACCCGCTTTACCAATGAGGGGGCCTTGCCCGCTGGGCTGA
CAGGCGATTCGCAAGCCTATGACGTCTGGCTTCGGGATAAGGTCCGCGAAGCTATGGAGGACCAGCGGCCGCCTGTTTCC
CATGCAGACGCCCACGCTCGCATGGCAGATATCAAGGCCCAGGTTCTGGCGCGCCGCGATGCGAAGGGCCAATGA

Structures

Toxin

• AlphaFold DB: E8RRF7

Antitoxin

• AlphaFold DB: E8RRF8