Detailed information of TA system TA025003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 703900..705415
• Replicon: chromosome
• Accession: NC_014816
• Organism: Asticcacaulis excentricus CB 48

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: E8RRM7
• Locus tag: ASTEX_RS03275
• Protein ID: WP_013478182.1
• Coordinates: 703900..704775 (-)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: ASTEX_RS03280
• Protein ID: WP_049781592.1
• Coordinates: 704768..705415 (-)
• Length: 216 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASTEX_RS03250	699371..701437	-	2067	WP_013478178.1	potassium-transporting ATPase subunit KdpB	-
ASTEX_RS03255	701442..703139	-	1698	WP_041658822.1	potassium-transporting ATPase subunit KdpA	-
ASTEX_RS03260	703167..703259	-	93	WP_013478180.1	K(+)-transporting ATPase subunit F	-
ASTEX_RS03275	703900..704775	-	876	WP_013478182.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ASTEX_RS03280	704768..705415	-	648	WP_049781592.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
ASTEX_RS20630	705405..705698	-	294	WP_041658487.1	hypothetical protein	-
ASTEX_RS03290	705887..706918	+	1032	WP_013478184.1	tRNA dihydrouridine(20/20a) synthase DusA	-
ASTEX_RS03295	706902..707429	-	528	WP_041658825.1	TIGR02281 family clan AA aspartic protease	-
ASTEX_RS03300	708006..708380	-	375	WP_013478187.1	hypothetical protein	-
ASTEX_RS03305	708607..708969	-	363	WP_013478188.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-271)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (29-64)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 32523.25 Da
• Isoelectric Point: 4.9222

>T25003 WP_013478182.1 NC_014816:c704775-703900 [Asticcacaulis excentricus CB 48]
MDKLKANRAASVKDKLLRLAKAQGQPFDVMLVRYGLERLLYRLSISRYRNRFILKGGLLVTLWVDDAMRVTRDVDLLGQG
EDNQDLLKGTFSEILSIEVDDGLIFDSANLIAEAIREDIKYGGTRLKTLAFLERTRISITIDIGFGDAVTPAPMDIDFPN
LLDNEVSNVRAYPPTTVIAEKFQAMVNLGLINGRMKDYYDLWAIPQSLSIDQNALDAAIEATFSRRGTAVPTETPPGLSD
AMYTSPDKLRQWDAYARSIAFDGPPFREIVQTIWELVGSACSRINANAIRN

Nucleotide

• Length: 876 bp

>T25003 NC_014816:c704775-703900 [Asticcacaulis excentricus CB 48]
ATGGATAAGCTCAAAGCGAACAGGGCGGCCTCAGTAAAAGACAAGCTCCTAAGGCTCGCAAAAGCCCAGGGGCAGCCATT
CGACGTGATGCTGGTCAGATACGGCCTGGAGCGACTTCTTTATCGCCTTTCGATCTCAAGATATCGGAACCGATTCATCC
TGAAGGGCGGCCTGCTTGTAACTCTTTGGGTGGATGACGCTATGCGCGTCACCAGAGACGTGGATCTGCTTGGCCAGGGC
GAGGATAATCAAGACCTCTTAAAGGGAACATTCTCTGAGATATTGTCGATTGAAGTTGACGACGGCCTGATATTTGACTC
TGCGAACTTAATCGCCGAGGCGATCCGCGAGGATATTAAATACGGTGGCACCCGCCTTAAGACCTTAGCGTTCCTTGAGA
GGACACGCATTTCGATCACTATCGACATTGGTTTTGGAGATGCGGTTACGCCAGCGCCCATGGATATCGACTTCCCCAAC
CTGCTGGATAACGAAGTATCAAACGTCAGAGCTTATCCTCCCACAACGGTTATCGCCGAAAAATTCCAGGCCATGGTCAA
CCTCGGCCTAATCAACGGCCGAATGAAGGACTATTACGATCTTTGGGCCATTCCGCAGAGCCTGAGCATCGATCAGAATG
CACTGGACGCAGCAATTGAAGCTACGTTCAGCCGCCGCGGCACAGCCGTACCAACTGAGACGCCTCCTGGCCTTTCAGAC
GCAATGTACACATCTCCCGATAAGCTTCGCCAATGGGATGCCTATGCACGTTCCATTGCGTTTGATGGCCCGCCTTTCCG
GGAGATCGTCCAGACGATTTGGGAACTGGTGGGCTCCGCTTGCTCACGCATCAATGCCAACGCCATTCGCAACTAA

Antitoxin

Protein

• Length: 216 a.a.
• Molecular weight: 23662.30 Da
• Isoelectric Point: 10.4280

>AT25003 WP_049781592.1 NC_014816:c705415-704768 [Asticcacaulis excentricus CB 48]
MRISGRFCIVEPPALTQQQRLNAIVKARPLVRAHELRAQGITAATISRAEASGLIRRVSRGLYQSLDSDIDTNQPLAEIS
KQIPNGIICMTSALAYHGLTDQMPRRIWVAIAKDTWAPTVSYPPMRLVRFKAASIRDGIEYHLISGVEVPIYSVARTLAD
VFRNPRLVDRSVAIESLKSALSQRKATPSEIYEAARHGGTSKTIAPYLEALTSNG

Nucleotide

• Length: 648 bp

>AT25003 NC_014816:c705415-704768 [Asticcacaulis excentricus CB 48]
ATGAGGATTAGTGGTAGGTTTTGTATCGTGGAGCCCCCTGCCCTTACACAGCAGCAACGCCTGAATGCTATCGTCAAGGC
CCGTCCGCTTGTGCGCGCTCACGAGCTAAGGGCCCAAGGCATCACGGCTGCAACTATCTCACGCGCGGAGGCAAGTGGTC
TGATAAGGCGGGTTTCGCGCGGCCTGTACCAAAGTCTGGACAGCGATATAGATACCAACCAGCCCCTTGCCGAAATTTCC
AAGCAAATTCCCAATGGCATTATCTGTATGACATCCGCACTGGCGTACCATGGCCTTACAGACCAGATGCCACGCAGAAT
ATGGGTCGCCATTGCTAAGGACACATGGGCACCGACCGTAAGTTACCCCCCGATGCGCCTGGTCCGCTTTAAAGCCGCCT
CAATCAGAGATGGCATAGAATACCATCTTATTTCAGGTGTTGAAGTTCCAATCTACTCCGTTGCCAGAACACTGGCTGAC
GTATTTCGCAACCCCCGTCTGGTTGATCGCTCCGTCGCGATAGAAAGTCTGAAATCTGCCCTGAGCCAACGTAAGGCAAC
CCCATCGGAAATTTACGAGGCTGCCAGGCATGGTGGCACGAGCAAGACGATAGCGCCCTATCTGGAAGCACTGACATCCA
ATGGATAA

Structures

Toxin

• AlphaFold DB: E8RRM7