Detailed information of TA system TA025001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 30562..31233
• Replicon: chromosome
• Accession: NC_014816
• Organism: Asticcacaulis excentricus CB 48

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ASTEX_RS00130
• Protein ID: WP_013477567.1
• Coordinates: 30820..31233 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E8RMW9
• Locus tag: ASTEX_RS00125
• Protein ID: WP_013477566.1
• Coordinates: 30562..30804 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASTEX_RS00105	25927..26484	-	558	WP_013477560.1	copper chaperone PCu(A)C	-
ASTEX_RS00110	26499..27494	-	996	WP_013477561.1	tryptophan--tRNA ligase	-
ASTEX_RS00115	27507..29030	-	1524	WP_144004663.1	murein biosynthesis integral membrane protein MurJ	-
ASTEX_RS00120	29533..29835	+	303	WP_013477563.1	DUF1778 domain-containing protein	-
ASTEX_RS19720	29838..30347	+	510	WP_013477564.1	GNAT family N-acetyltransferase	-
ASTEX_RS00125	30562..30804	+	243	WP_013477566.1	hypothetical protein	Antitoxin
ASTEX_RS00130	30820..31233	+	414	WP_013477567.1	type II toxin-antitoxin system VapC family toxin	Toxin
ASTEX_RS00135	31541..32821	-	1281	WP_013477568.1	type II toxin-antitoxin system HipA family toxin	-
ASTEX_RS00140	32826..33125	-	300	WP_041658656.1	helix-turn-helix domain-containing protein	-
ASTEX_RS00145	33405..33620	+	216	WP_013477570.1	type II toxin-antitoxin system ParD family antitoxin	-
ASTEX_RS00150	33685..35382	-	1698	WP_013477571.1	PAS domain-containing methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14703.83 Da
• Isoelectric Point: 6.4868

>T25001 WP_013477567.1 NC_014816:30820-31233 [Asticcacaulis excentricus CB 48]
VIILDTNVVSELMRPEPHEHVRDWLRRSGDVRLTTTAITVAEITHGLERLPDGRRKSDLQAAFVKLLDALGALPLDDAAG
FKAGKFRALREAGGHPSSPSDMMIAGIAVASGASLATRNTKDFEALPLALIDPWAAN

Nucleotide

• Length: 414 bp

>T25001 NC_014816:30820-31233 [Asticcacaulis excentricus CB 48]
GTGATCATTCTCGACACCAATGTTGTATCAGAGCTGATGAGACCGGAGCCGCATGAGCATGTCCGAGATTGGCTGCGACG
ATCGGGTGACGTAAGGCTTACGACAACGGCTATAACGGTTGCGGAAATAACTCATGGGTTGGAGCGGTTGCCGGATGGCA
GGCGCAAGAGTGATTTGCAGGCTGCCTTCGTAAAGTTATTGGACGCCCTCGGCGCATTACCATTGGACGACGCGGCTGGA
TTCAAGGCTGGGAAGTTTCGAGCTTTGCGTGAAGCTGGAGGACACCCGTCTAGTCCATCGGATATGATGATTGCGGGGAT
CGCCGTTGCGTCGGGAGCCTCTCTTGCAACGCGCAACACTAAGGACTTCGAGGCGCTACCCTTAGCGCTGATAGATCCGT
GGGCCGCCAATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8559.60 Da
• Isoelectric Point: 5.6401

>AT25001 WP_013477566.1 NC_014816:30562-30804 [Asticcacaulis excentricus CB 48]
MASITVRNIDDTLKERLRVRAATHGHSMEEEVRQILSQAVGGLTGAGLLSLSRDLFSGNSGVDLDLPSRPQDRAAPEFGA

Nucleotide

• Length: 243 bp

>AT25001 NC_014816:30562-30804 [Asticcacaulis excentricus CB 48]
ATGGCGAGCATCACCGTCCGCAATATTGATGACACGCTGAAAGAACGCCTTCGCGTTCGCGCAGCAACGCACGGCCATTC
TATGGAAGAGGAAGTCCGACAAATACTGAGCCAGGCGGTTGGTGGGCTCACGGGTGCTGGCTTACTTTCTCTGTCGAGGG
ATTTGTTCAGCGGCAATAGCGGCGTAGATCTCGATCTGCCTTCCCGACCGCAAGATCGGGCAGCGCCTGAATTTGGCGCT
TAA

Structures

Antitoxin

• AlphaFold DB: E8RMW9