Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3731252..3731877 Replicon chromosome
Accession NC_014815
Organism Micromonospora sp. L5

Toxin (Protein)


Gene name vapC Uniprot ID A0A1C6TLL3
Locus tag ML5_RS17685 Protein ID WP_013475595.1
Coordinates 3731252..3731671 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3M9KLX8
Locus tag ML5_RS17690 Protein ID WP_013475596.1
Coordinates 3731668..3731877 (-) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ML5_RS17650 3727229..3728368 - 1140 WP_013475593.1 site-2 protease family protein -
ML5_RS17655 3728463..3728732 + 270 WP_174361730.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
ML5_RS17660 3728729..3729010 + 282 WP_083395318.1 type II toxin-antitoxin system RelE/ParE family toxin -
ML5_RS17665 3729066..3729266 - 201 WP_013287873.1 DUF397 domain-containing protein -
ML5_RS17670 3729259..3730137 - 879 WP_174361729.1 helix-turn-helix domain-containing protein -
ML5_RS17675 3730504..3730818 + 315 WP_013475594.1 DivIVA domain-containing protein -
ML5_RS17680 3730815..3731069 + 255 WP_013287870.1 hypothetical protein -
ML5_RS17685 3731252..3731671 - 420 WP_013475595.1 PIN domain nuclease Toxin
ML5_RS17690 3731668..3731877 - 210 WP_013475596.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
ML5_RS17695 3731966..3732817 - 852 WP_013475597.1 alpha/beta fold hydrolase -
ML5_RS17700 3732957..3733700 + 744 WP_013287866.1 3-oxoacyl-ACP reductase FabG -
ML5_RS17705 3733741..3734631 - 891 WP_013475598.1 L,D-transpeptidase -
ML5_RS17710 3734705..3736150 - 1446 WP_013475599.1 alpha-amylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-119)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15376.68 Da        Isoelectric Point: 6.6416

>T25000 WP_013475595.1 NC_014815:c3731671-3731252 [Micromonospora sp. L5]
MTRERYLLDKSALARWPKPAVAPVLDELSDRGLLAVCGAIEIEVVHSARSAKDAKRARWLLRGFDWLAMPDDIWDRAIDI
QVQALHKGNHRALSMADLLIAATAERHGVTVLHYDGDFDLITAITGQPTTWVVPAGKAD

Download         Length: 420 bp

>T25000 NC_014815:c3731671-3731252 [Micromonospora sp. L5]
ATGACGCGGGAGCGCTACCTCCTCGACAAGTCGGCGCTGGCCCGGTGGCCGAAGCCCGCCGTGGCGCCGGTCCTGGACGA
GTTGTCCGATCGCGGTCTGCTGGCGGTCTGCGGCGCCATCGAGATCGAGGTCGTACACAGCGCCCGCTCGGCCAAGGACG
CGAAGCGCGCTCGATGGCTGCTCCGCGGTTTCGACTGGCTCGCCATGCCCGACGACATCTGGGATCGTGCGATCGACATC
CAGGTGCAGGCGCTGCACAAGGGCAACCACCGCGCGCTCTCGATGGCCGATCTGCTGATCGCCGCGACGGCCGAACGGCA
TGGCGTGACCGTTCTGCACTACGACGGTGACTTCGACCTGATCACCGCGATCACCGGCCAGCCGACGACGTGGGTCGTCC
CGGCCGGGAAGGCCGACTGA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 7395.28 Da        Isoelectric Point: 4.3719

>AT25000 WP_013475596.1 NC_014815:c3731877-3731668 [Micromonospora sp. L5]
MSRTILDVDDDLLAEAAEILGTGTKKATVNAALQEVVNREKRREFADWLKSGGLPDLTDPGQASKPEAA

Download         Length: 210 bp

>AT25000 NC_014815:c3731877-3731668 [Micromonospora sp. L5]
ATGTCCCGCACCATTCTCGATGTCGACGACGACCTCCTGGCCGAGGCCGCCGAGATCCTCGGCACGGGCACCAAGAAGGC
CACTGTCAACGCCGCGCTGCAGGAAGTGGTCAACCGGGAGAAGCGCCGGGAGTTCGCCGACTGGCTCAAGAGCGGCGGCC
TGCCGGACCTCACCGACCCGGGGCAGGCGTCGAAGCCCGAAGCGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C6TLL3

Antitoxin

Source ID Structure
AlphaFold DB A0A3M9KLX8

References