Detailed information of TA system TA024998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4808684..4809302
• Replicon: chromosome
• Accession: NC_014814
• Organism: Mycobacterium gilvum Spyr1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E6TGL2
• Locus tag: MSPYR1_RS22760
• Protein ID: WP_013472826.1
• Coordinates: 4808684..4809085 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MSPYR1_RS22765
• Protein ID: WP_013472827.1
• Coordinates: 4809072..4809302 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSPYR1_RS27910	4804071..4804481	-	411	WP_013472821.1	PIN domain-containing protein	-
MSPYR1_RS27915	4804488..4804727	-	240	WP_013472822.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MSPYR1_RS22740	4804833..4805966	-	1134	WP_013472823.1	hypothetical protein	-
MSPYR1_RS22745	4806087..4806476	+	390	WP_011896019.1	ParD-like family protein	-
MSPYR1_RS22750	4806511..4807092	+	582	WP_013472824.1	zeta toxin family protein	-
MSPYR1_RS22755	4807170..4808585	+	1416	WP_158022643.1	plasmid pRiA4b ORF-3 family protein	-
MSPYR1_RS22760	4808684..4809085	-	402	WP_013472826.1	type II toxin-antitoxin system VapC family toxin	Toxin
MSPYR1_RS22765	4809072..4809302	-	231	WP_013472827.1	hypothetical protein	Antitoxin
MSPYR1_RS22770	4809607..4812360	-	2754	WP_013472828.1	AAA family ATPase	-
MSPYR1_RS22775	4812364..4813302	-	939	WP_013472829.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4804833..4840220	35387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14146.36 Da
• Isoelectric Point: 5.7429

>T24998 WP_013472826.1 NC_014814:c4809085-4808684 [Mycolicibacterium gilvum Spyr1]
VIVVDASVLAVALGDDGADGRLARQRLTGEALAAPELIDLEVASVWRRHVAAKLMTARRADAAVSDLADLPLRRCSHRPL
LERIWVLRRTVTPYDAAYAALAEALEVVLVTADVRLARAPGLACDVEVIGSSG

Nucleotide

• Length: 402 bp

>T24998 NC_014814:c4809085-4808684 [Mycolicibacterium gilvum Spyr1]
GTGATCGTCGTTGACGCGAGCGTCTTGGCGGTGGCGCTGGGCGATGACGGCGCGGATGGCCGTCTCGCCCGTCAGCGTTT
GACTGGCGAGGCGCTGGCAGCGCCTGAGCTCATCGATCTCGAGGTCGCTTCTGTCTGGCGGCGTCACGTCGCGGCCAAGC
TGATGACCGCTCGACGCGCCGACGCTGCAGTATCGGATCTTGCGGACCTGCCACTGCGGCGATGCTCGCATCGTCCACTG
CTGGAACGTATCTGGGTGCTGCGTCGCACGGTCACGCCGTATGACGCGGCGTATGCCGCGCTCGCCGAAGCCCTCGAAGT
GGTTTTGGTGACTGCCGATGTACGACTCGCGCGAGCGCCGGGCCTCGCCTGTGATGTCGAGGTAATCGGAAGCTCCGGCT
GA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8658.73 Da
• Isoelectric Point: 10.0732

>AT24998 WP_013472827.1 NC_014814:c4809302-4809072 [Mycolicibacterium gilvum Spyr1]
MPSVQIKDVPDDTHRVLRERAARAHQSLQEYLRSRLIAEASQPTAEEVFERAAVRKGGKVSFSAAVDEIRAERDRR

Nucleotide

• Length: 231 bp

>AT24998 NC_014814:c4809302-4809072 [Mycolicibacterium gilvum Spyr1]
ATGCCCAGTGTGCAGATCAAAGACGTCCCGGATGACACACACCGGGTCTTGCGGGAGCGGGCGGCGCGGGCGCATCAATC
GCTTCAGGAGTACCTTCGCAGCCGACTGATCGCCGAGGCGAGTCAGCCCACTGCTGAAGAGGTCTTCGAACGTGCCGCCG
TGCGCAAAGGCGGAAAGGTGTCGTTCAGTGCGGCGGTCGATGAGATCCGGGCCGAACGTGATCGTCGTTGA

Structures

Toxin

• AlphaFold DB: E6TGL2