Detailed information of TA system TA024997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 4806087..4807092
• Replicon: chromosome
• Accession: NC_014814
• Organism: Mycobacterium gilvum Spyr1

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: E6TGL0
• Locus tag: MSPYR1_RS22750
• Protein ID: WP_013472824.1
• Coordinates: 4806511..4807092 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: E6TGK9
• Locus tag: MSPYR1_RS22745
• Protein ID: WP_011896019.1
• Coordinates: 4806087..4806476 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSPYR1_RS22725	4801497..4804022	+	2526	WP_013472820.1	SEC-C domain-containing protein	-
MSPYR1_RS27910	4804071..4804481	-	411	WP_013472821.1	PIN domain-containing protein	-
MSPYR1_RS27915	4804488..4804727	-	240	WP_013472822.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MSPYR1_RS22740	4804833..4805966	-	1134	WP_013472823.1	hypothetical protein	-
MSPYR1_RS22745	4806087..4806476	+	390	WP_011896019.1	ParD-like family protein	Antitoxin
MSPYR1_RS22750	4806511..4807092	+	582	WP_013472824.1	zeta toxin family protein	Toxin
MSPYR1_RS22755	4807170..4808585	+	1416	WP_158022643.1	plasmid pRiA4b ORF-3 family protein	-
MSPYR1_RS22760	4808684..4809085	-	402	WP_013472826.1	type II toxin-antitoxin system VapC family toxin	-
MSPYR1_RS22765	4809072..4809302	-	231	WP_013472827.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4804833..4840220	35387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-144)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21063.97 Da
• Isoelectric Point: 8.5027

>T24997 WP_013472824.1 NC_014814:4806511-4807092 [Mycolicibacterium gilvum Spyr1]
VKRLDLVVGSNGAGKSTFIELTLAPLVPRSVYVNADEIAKRRWPDDPARHSYEAARIAANTRARLIELGESFIAETVFSH
PSKLELLDVAHAAGYTIILHAAMIPEELAVQRVRHRVQAGGHDVPESKIRQQYQRLWDVVATAAGRADEATFYDNSAIRG
PRIVVQLAAGIAVGSALWPDWTPAPLTSRWAGG

Nucleotide

• Length: 582 bp

>T24997 NC_014814:4806511-4807092 [Mycolicibacterium gilvum Spyr1]
GTGAAGCGCCTCGACCTCGTCGTCGGATCCAATGGCGCGGGCAAATCCACGTTCATCGAGCTGACCCTCGCGCCGCTGGT
GCCGCGCAGCGTCTACGTGAACGCCGATGAGATCGCCAAGCGACGCTGGCCCGACGATCCAGCCAGGCATAGCTACGAGG
CAGCGCGGATTGCCGCCAACACTCGCGCAAGGCTCATCGAACTCGGCGAGTCCTTCATCGCCGAGACCGTGTTCTCTCAC
CCCTCGAAACTTGAACTTCTCGACGTCGCCCACGCAGCGGGCTACACGATCATCCTGCACGCAGCGATGATCCCGGAAGA
GCTTGCCGTGCAACGTGTCCGACATCGCGTGCAGGCGGGAGGCCACGACGTCCCCGAATCCAAAATCCGGCAGCAATATC
AACGGCTCTGGGATGTGGTCGCCACCGCGGCCGGCCGTGCGGACGAGGCAACGTTCTACGACAACAGCGCGATCCGGGGT
CCACGCATCGTCGTTCAGCTCGCTGCCGGCATCGCAGTGGGTTCGGCCTTGTGGCCTGACTGGACGCCGGCCCCGCTCAC
CAGCCGTTGGGCAGGCGGGTGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13600.10 Da
• Isoelectric Point: 4.9205

>AT24997 WP_011896019.1 NC_014814:4806087-4806476 [Mycolicibacterium gilvum Spyr1]
MSNTADRVTRIAADLMDSAAAEGARQSRSAKQQLDHWARVGRAVSSQHSVARRKVEAALAGDVPLRDLTDEEGVVFNAEI
SAAIQERLVRADYGAVLAARGVTTVALDEDGEIVQYAPDGSSVRLGPGA

Nucleotide

• Length: 390 bp

>AT24997 NC_014814:4806087-4806476 [Mycolicibacterium gilvum Spyr1]
ATGTCGAACACTGCCGACCGGGTGACCAGGATCGCAGCGGACCTGATGGACAGCGCCGCTGCCGAAGGCGCACGTCAGAG
CAGGTCGGCGAAACAACAGCTGGACCACTGGGCGCGGGTCGGCCGCGCGGTGTCCAGTCAGCACAGCGTGGCGCGCCGCA
AGGTCGAAGCTGCCCTCGCCGGCGATGTTCCTCTACGGGATCTGACCGACGAGGAAGGTGTCGTCTTCAACGCCGAGATC
TCCGCGGCGATCCAGGAACGGCTCGTGAGGGCCGATTACGGCGCGGTGTTGGCCGCGCGAGGAGTCACGACGGTGGCGCT
CGACGAGGACGGCGAGATCGTTCAGTACGCACCAGACGGCAGTTCGGTACGGCTGGGCCCCGGAGCGTGA

Structures

Toxin

• AlphaFold DB: E6TGL0

Antitoxin

• AlphaFold DB: E6TGK9