Detailed information of TA system TA024995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4794020..4794651
• Replicon: chromosome
• Accession: NC_014814
• Organism: Mycobacterium gilvum Spyr1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E6TGJ9
• Locus tag: MSPYR1_RS22695
• Protein ID: WP_013472816.1
• Coordinates: 4794238..4794651 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E6TGJ8
• Locus tag: MSPYR1_RS22690
• Protein ID: WP_013472815.1
• Coordinates: 4794020..4794247 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSPYR1_RS22675	4790060..4791790	-	1731	WP_013472812.1	VanW family protein	-
MSPYR1_RS22680	4792274..4792849	+	576	WP_013472813.1	hypothetical protein	-
MSPYR1_RS22685	4792954..4793931	+	978	WP_013472814.1	Lsr2 family protein	-
MSPYR1_RS22690	4794020..4794247	+	228	WP_013472815.1	hypothetical protein	Antitoxin
MSPYR1_RS22695	4794238..4794651	+	414	WP_013472816.1	PIN domain nuclease	Toxin
MSPYR1_RS22700	4794660..4795469	-	810	WP_011896013.1	DUF1206 domain-containing protein	-
MSPYR1_RS22705	4795549..4797804	-	2256	WP_013472817.1	peptidase S15	-
MSPYR1_RS22710	4798033..4799271	+	1239	WP_013472818.1	divalent metal cation transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (25-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15189.61 Da
• Isoelectric Point: 6.2331

>T24995 WP_013472816.1 NC_014814:4794238-4794651 [Mycolicibacterium gilvum Spyr1]
VALRPWLIDKSAYTRLAVSPDVELWMERIDRGLVRISTVTRLEIGYSFRTAAQARSEVASPPLTLMPIEYMTPTVEDRAR
EIQLTLADRGMHRAPSIPDLLVAATAEQAGLTVLHLHKDFDLIAGITGQPVEALRMG

Nucleotide

• Length: 414 bp

>T24995 NC_014814:4794238-4794651 [Mycolicibacterium gilvum Spyr1]
GTGGCACTGAGGCCATGGCTGATCGACAAGTCGGCATACACGCGGCTCGCTGTGTCACCAGACGTCGAACTCTGGATGGA
GCGCATCGACCGCGGGCTGGTGCGCATCAGCACGGTCACGCGTCTTGAGATCGGATATTCGTTTCGCACAGCAGCGCAGG
CGCGTTCCGAGGTCGCTTCGCCGCCCTTGACACTCATGCCCATCGAGTACATGACACCGACCGTCGAAGATCGCGCCCGG
GAAATACAACTCACCCTCGCCGACCGCGGCATGCACCGCGCACCATCGATACCCGACCTCCTCGTCGCCGCCACAGCAGA
ACAGGCCGGATTGACCGTCCTCCACCTCCACAAGGACTTCGACCTGATTGCCGGCATCACCGGACAACCCGTCGAGGCGC
TGCGAATGGGGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8119.96 Da
• Isoelectric Point: 5.0481

>AT24995 WP_013472815.1 NC_014814:4794020-4794247 [Mycolicibacterium gilvum Spyr1]
MADVLIRGLSEAAVAHIDAAAAAQGLSRQEYLRRRFEAERPRSDSGGRLTIDDLRRASAAASDLDNPEAMDGAWH

Nucleotide

• Length: 228 bp

>AT24995 NC_014814:4794020-4794247 [Mycolicibacterium gilvum Spyr1]
ATGGCTGACGTGTTAATCCGAGGGCTGTCGGAGGCAGCGGTGGCGCATATCGATGCCGCCGCAGCGGCGCAGGGCTTATC
GCGACAGGAGTATCTCCGGCGGCGCTTCGAGGCCGAACGTCCGCGCAGCGACTCCGGCGGTCGGTTGACGATCGACGATT
TGCGTCGCGCGTCGGCTGCGGCCTCTGACCTCGACAACCCCGAGGCGATGGACGGCGCGTGGCACTGA

Structures

Toxin

• AlphaFold DB: E6TGJ9

Antitoxin

• AlphaFold DB: E6TGJ8