Detailed information of TA system TA024994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1529223..1529767
• Replicon: chromosome
• Accession: NC_014814
• Organism: Mycobacterium gilvum Spyr1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E6TL20
• Locus tag: MSPYR1_RS07400
• Protein ID: WP_013471060.1
• Coordinates: 1529504..1529767 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E6TL19
• Locus tag: MSPYR1_RS07395
• Protein ID: WP_013471059.1
• Coordinates: 1529223..1529507 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSPYR1_RS07380	1525106..1526885	+	1780	Protein_1477	penicillin-binding protein	-
MSPYR1_RS07385	1526882..1528009	-	1128	WP_013471057.1	hypothetical protein	-
MSPYR1_RS07390	1528092..1529165	+	1074	WP_041787750.1	redox-regulated ATPase YchF	-
MSPYR1_RS07395	1529223..1529507	+	285	WP_013471059.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MSPYR1_RS07400	1529504..1529767	+	264	WP_013471060.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MSPYR1_RS07405	1529863..1530570	+	708	WP_013471061.1	GNAT family N-acetyltransferase	-
MSPYR1_RS07410	1530666..1531526	+	861	WP_011892973.1	DUF4344 domain-containing metallopeptidase	-
MSPYR1_RS07415	1531530..1532024	-	495	WP_011892974.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
MSPYR1_RS07420	1532034..1532255	-	222	WP_041788453.1	hypothetical protein	-
MSPYR1_RS07425	1532497..1532946	+	450	WP_013471063.1	hypothetical protein	-
MSPYR1_RS07430	1532955..1533602	-	648	WP_013471064.1	hypothetical protein	-
MSPYR1_RS07435	1533718..1534410	-	693	WP_011892978.1	guanylate cyclase	-
MSPYR1_RS28710	1534438..1534599	+	162	WP_011892979.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (34-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9962.43 Da
• Isoelectric Point: 10.1896

>T24994 WP_013471060.1 NC_014814:1529504-1529767 [Mycolicibacterium gilvum Spyr1]
VTYVVVLSPGARRALTEQLPEAVAAACFAFIYGPLAENPRRVGKQLRNELAGTYSARRGEFRVIYDIDDNRIRVEVISIR
HRRDVYR

Nucleotide

• Length: 264 bp

>T24994 NC_014814:1529504-1529767 [Mycolicibacterium gilvum Spyr1]
GTGACGTATGTAGTGGTGTTGTCTCCTGGGGCACGCAGGGCTCTAACAGAACAGCTTCCGGAGGCGGTCGCTGCGGCCTG
TTTCGCGTTCATCTACGGACCACTGGCCGAAAATCCCCGCCGCGTCGGTAAGCAACTGCGAAACGAGCTGGCCGGTACCT
ATTCGGCGCGGCGAGGCGAGTTCCGGGTCATCTACGACATCGACGACAACCGGATTCGAGTGGAGGTCATCTCGATTCGT
CACCGCAGGGACGTGTACCGCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10691.13 Da
• Isoelectric Point: 5.1875

>AT24994 WP_013471059.1 NC_014814:1529223-1529507 [Mycolicibacterium gilvum Spyr1]
MTTLPLAEVRANLSKLVDEAVRTHQRIEVTRQGRRAAVILSAEDYDSIMETLDILSDRELMKELRQGQEDVARGNFHTLD
DVAKEMRATGRLPQ

Nucleotide

• Length: 285 bp

>AT24994 NC_014814:1529223-1529507 [Mycolicibacterium gilvum Spyr1]
ATGACGACGCTGCCGTTGGCTGAGGTCCGCGCGAATCTGTCGAAGCTCGTCGATGAAGCGGTGCGAACACACCAACGGAT
CGAGGTGACTCGGCAAGGACGTCGTGCCGCAGTGATCCTGAGCGCCGAGGACTACGACTCGATCATGGAGACGCTCGATA
TTTTGAGCGATCGCGAACTGATGAAGGAACTCCGGCAGGGGCAGGAAGACGTCGCGCGCGGGAACTTTCACACGCTCGAC
GACGTGGCGAAGGAGATGCGAGCCACGGGCCGACTGCCCCAGTGA

Structures

Toxin

• AlphaFold DB: E6TL20

Antitoxin

• AlphaFold DB: E6TL19