Detailed information of TA system TA024987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 32453..33039
• Replicon: plasmid pSULKU03
• Accession: NC_014756
• Organism: Sulfuricurvum kujiense DSM 16994

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E4U423
• Locus tag: SULKU_RS14025
• Protein ID: WP_013450047.1
• Coordinates: 32749..33039 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E4U422
• Locus tag: SULKU_RS14020
• Protein ID: WP_013450046.1
• Coordinates: 32453..32746 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SULKU_RS14005	27793..28677	-	885	WP_013450043.1	diguanylate cyclase	-
SULKU_RS14010	28755..31673	-	2919	WP_013450044.1	Tn3 family transposase	-
SULKU_RS14015	31829..32452	+	624	WP_013450045.1	recombinase family protein	-
SULKU_RS14020	32453..32746	-	294	WP_013450046.1	putative addiction module antidote protein	Antitoxin
SULKU_RS14025	32749..33039	-	291	WP_013450047.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SULKU_RS14030	33374..33748	-	375	WP_013450048.1	S24 family peptidase	-
SULKU_RS14035	33926..35062	-	1137	WP_013450049.1	site-specific integrase	-
SULKU_RS14040	35063..35302	-	240	WP_013450050.1	hypothetical protein	-
SULKU_RS14045	35302..35700	-	399	WP_013450051.1	type II toxin-antitoxin system VapC family toxin	-
SULKU_RS14050	35697..35942	-	246	WP_013450052.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
SULKU_RS14055	36493..36813	+	321	WP_013450053.1	hypothetical protein	-
SULKU_RS14060	36817..37578	-	762	WP_013450054.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51014	51014
	-	flank	IS/Tn	-	-	31829..32452	623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11102.90 Da
• Isoelectric Point: 9.4070

>T24987 WP_013450047.1 NC_014756:c33039-32749 [Sulfuricurvum kujiense DSM 16994]
MYNIQQTQKFSQWLIKLKDMRARIAIARRIERAQCGNLGDAKSVGESVYEMRIDMGPGYRLYYTMRGDEIIVLLVGGDKS
TQQRDIEKAIEMAKEI

Nucleotide

• Length: 291 bp

>T24987 NC_014756:c33039-32749 [Sulfuricurvum kujiense DSM 16994]
ATGTATAACATTCAACAAACCCAAAAGTTTTCACAATGGCTGATAAAGCTAAAAGATATGCGAGCCCGTATCGCAATCGC
ACGTCGTATTGAACGTGCTCAATGTGGTAATCTTGGGGATGCTAAAAGTGTTGGTGAAAGTGTTTACGAGATGCGTATCG
ATATGGGTCCGGGTTATCGGCTCTATTATACGATGCGTGGAGACGAAATAATTGTCTTGCTTGTTGGTGGCGATAAGTCG
ACTCAACAACGCGATATTGAAAAAGCCATAGAGATGGCAAAGGAGATATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10739.19 Da
• Isoelectric Point: 4.8129

>AT24987 WP_013450046.1 NC_014756:c32746-32453 [Sulfuricurvum kujiense DSM 16994]
MELTLTPFDMTQYLDSEEAIAEYLTQVLEEGDTDELIRAIGHIAKARGMSQIAQESGLGRESLYKTFAPGSKPRFDTIAK
VVHSLGLHFQITPVRAS

Nucleotide

• Length: 294 bp

>AT24987 NC_014756:c32746-32453 [Sulfuricurvum kujiense DSM 16994]
ATGGAACTGACATTAACCCCATTTGATATGACACAGTATTTGGATAGCGAAGAGGCGATTGCCGAATATCTAACCCAAGT
CCTCGAAGAAGGTGATACCGATGAGCTGATCCGTGCTATCGGGCATATCGCAAAAGCCCGTGGGATGAGTCAAATCGCAC
AAGAGAGTGGTCTTGGTCGTGAAAGCCTCTATAAAACATTTGCACCCGGCTCAAAACCTCGTTTCGATACCATTGCAAAA
GTAGTGCATTCACTTGGATTGCATTTTCAGATTACACCGGTGCGGGCATCGTAA

Structures

Toxin

• AlphaFold DB: E4U423

Antitoxin

• AlphaFold DB: E4U422