Detailed information of TA system TA024982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-MazE
• Location: 146182..146777
• Replicon: plasmid pBRH02
• Accession: NC_014723
• Organism: Burkholderia rhizoxinica HKI 454

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: E5AW95
• Locus tag: RBRH_RS15940
• Protein ID: WP_013436802.1
• Coordinates: 146182..146532 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: E5AW96
• Locus tag: RBRH_RS15945
• Protein ID: WP_013436803.1
• Coordinates: 146526..146777 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBRH_RS19565	144226..144468	+	243	Protein_126	IS5/IS1182 family transposase	-
RBRH_RS19570	145299..145628	+	330	WP_157864649.1	hypothetical protein	-
RBRH_RS15935	145514..145981	-	468	WP_157864659.1	hypothetical protein	-
RBRH_RS15940	146182..146532	-	351	WP_013436802.1	type II toxin-antitoxin system ChpB family toxin	Toxin
RBRH_RS15945	146526..146777	-	252	WP_013436803.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RBRH_RS15950	147101..147445	+	345	WP_041755140.1	hypothetical protein	-
RBRH_RS19575	147591..148228	-	638	Protein_132	ATPase	-
RBRH_RS15960	148459..148865	-	407	Protein_133	type II toxin-antitoxin system VapC family toxin	-
RBRH_RS15965	148856..149110	-	255	WP_013436809.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
RBRH_RS15970	149716..149994	+	279	WP_041755167.1	hypothetical protein	-
RBRH_RS15975	149991..150290	+	300	WP_013436813.1	type II toxin-antitoxin system RelE/ParE family toxin	-
RBRH_RS18440	150811..150984	-	174	Protein_137	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..172525	172525
	-	flank	IS/Tn	-	-	144226..144384	158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-113)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12374.32 Da
• Isoelectric Point: 10.6530

>T24982 WP_013436802.1 NC_014723:c146532-146182 [Mycetohabitans rhizoxinica HKI 454]
MVRRVKFERGDIVRVSLNPTVGREQQGDFRPALVLSPAVFNALGVALVAPITQGGASARFAGFAVPLSGSGTETQGVALV
NMARMLDLEARGARKLERAPVEVVEDALARFQTIIE

Nucleotide

• Length: 351 bp

>T24982 NC_014723:c146532-146182 [Mycetohabitans rhizoxinica HKI 454]
TTGGTGAGGAGGGTCAAGTTCGAGCGCGGTGATATCGTGCGGGTGAGCCTAAACCCGACTGTCGGGCGAGAGCAGCAAGG
CGATTTTCGCCCGGCGCTCGTTCTATCTCCGGCGGTGTTCAACGCCTTGGGCGTGGCGCTCGTTGCCCCGATCACCCAAG
GCGGCGCATCGGCTCGCTTTGCTGGGTTCGCCGTACCGCTTTCCGGTTCTGGAACCGAGACGCAAGGTGTAGCGCTCGTC
AACATGGCGCGTATGCTCGACCTGGAGGCACGCGGCGCGCGTAAGCTCGAACGTGCGCCAGTTGAAGTGGTTGAGGACGC
GCTGGCTCGGTTTCAGACGATTATTGAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9160.51 Da
• Isoelectric Point: 5.0165

>AT24982 WP_013436803.1 NC_014723:c146777-146526 [Mycetohabitans rhizoxinica HKI 454]
MELKIQKWGNSAAVRLPSVLLEQINASIGSSLNADVRPDGVMLAPARRKYSLDDLVAQCDTKAFFPEDMAAWSEVKPVGR
EAW

Nucleotide

• Length: 252 bp

>AT24982 NC_014723:c146777-146526 [Mycetohabitans rhizoxinica HKI 454]
ATGGAACTCAAGATTCAAAAATGGGGCAATAGCGCGGCAGTTCGTTTGCCGAGTGTGCTGCTCGAGCAGATCAACGCGTC
GATCGGCAGTTCACTAAACGCCGACGTGCGTCCGGATGGCGTAATGCTGGCCCCAGCCCGCCGCAAGTATTCGCTGGATG
ATCTTGTTGCCCAATGCGACACGAAGGCTTTTTTTCCGGAGGACATGGCGGCATGGAGTGAGGTCAAACCGGTGGGGCGT
GAGGCTTGGTGA

Structures

Toxin

• AlphaFold DB: E5AW95

Antitoxin

• AlphaFold DB: E5AW96