Detailed information of TA system TA024981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 46866..47451
• Replicon: plasmid pBRH02
• Accession: NC_014723
• Organism: Burkholderia rhizoxinica HKI 454

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: RBRH_RS15495
• Protein ID: WP_013436686.1
• Coordinates: 46866..47153 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: E5AVY0
• Locus tag: RBRH_RS15500
• Protein ID: WP_013436687.1
• Coordinates: 47155..47451 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBRH_RS15470	42464..43584	+	1121	WP_157864640.1	IS3 family transposase	-
RBRH_RS15480	44424..45065	+	642	WP_041755152.1	DNA methyltransferase	-
RBRH_RS15485	45587..45787	+	201	WP_013436683.1	hypothetical protein	-
RBRH_RS15490	45800..46339	+	540	WP_013436684.1	hypothetical protein	-
RBRH_RS15495	46866..47153	+	288	WP_013436686.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RBRH_RS15500	47155..47451	+	297	WP_013436687.1	putative addiction module antidote protein	Antitoxin
RBRH_RS15505	47734..48003	-	270	WP_013436688.1	hypothetical protein	-
RBRH_RS15510	48316..48708	+	393	WP_013436689.1	hypothetical protein	-
RBRH_RS15515	48695..50719	+	2025	WP_013436690.1	type IV secretion system DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..172525	172525

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Uncharacterized protein HI1420 (4-94)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10684.42 Da
• Isoelectric Point: 10.2286

>T24981 WP_013436686.1 NC_014723:46866-47153 [Mycetohabitans rhizoxinica HKI 454]
MSRTAEFAKWMRDLRDPIARAQIAKRIQRLARGQFGDVEPVGSCVSEMRVHTGPGYRVYFTQCGSALIILLCGGDKSTQQ
RDIKRALMLAAELRD

Nucleotide

• Length: 288 bp

>T24981 NC_014723:46866-47153 [Mycetohabitans rhizoxinica HKI 454]
TTGAGCAGAACTGCCGAATTTGCCAAGTGGATGCGTGACTTGCGTGATCCCATCGCTCGTGCACAGATCGCCAAGCGGAT
TCAGCGGCTGGCGCGTGGACAGTTTGGTGATGTGGAACCGGTTGGCTCTTGCGTCAGCGAAATGCGCGTGCATACTGGGC
CTGGCTATCGCGTCTACTTTACCCAATGCGGTTCAGCGCTCATCATACTGTTATGCGGTGGCGACAAATCAACGCAACAA
CGGGACATTAAGCGAGCCTTGATGCTTGCTGCTGAATTGAGAGATTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10496.81 Da
• Isoelectric Point: 4.5837

>AT24981 WP_013436687.1 NC_014723:47155-47451 [Mycetohabitans rhizoxinica HKI 454]
MGIKTTPFDAADYLDVPEAQAEYLRVSFETGDAADIQEAIGTVARARGMTQIARAAGVGRESLYKALDEHGNPEFATILS
VVRALGLKLTVTPTYDHA

Nucleotide

• Length: 297 bp

>AT24981 NC_014723:47155-47451 [Mycetohabitans rhizoxinica HKI 454]
ATGGGAATTAAGACGACACCTTTCGATGCGGCTGACTATCTTGACGTGCCCGAGGCGCAAGCTGAATATCTTCGCGTCTC
GTTCGAAACGGGCGACGCGGCCGACATTCAGGAAGCCATCGGTACTGTCGCGCGTGCGCGCGGCATGACGCAAATTGCGC
GCGCGGCGGGGGTTGGCCGTGAGAGCTTGTACAAGGCGCTAGATGAGCACGGCAATCCGGAATTCGCGACTATTCTAAGT
GTCGTGCGTGCGCTTGGGCTGAAACTCACTGTCACCCCTACCTACGATCATGCTTGA

Structures

Antitoxin

• AlphaFold DB: E5AVY0