Detailed information of TA system TA024976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1739751..1740505
• Replicon: chromosome
• Accession: NC_014722
• Organism: Burkholderia rhizoxinica HKI 454

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: E5ARW5
• Locus tag: RBRH_RS07670
• Protein ID: WP_013435576.1
• Coordinates: 1740008..1740505 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: RBRH_RS07665
• Protein ID: WP_041753630.1
• Coordinates: 1739751..1740011 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBRH_RS07645	1736302..1737280	-	979	Protein_1528	tRNA dihydrouridine(20/20a) synthase DusA	-
RBRH_RS07650	1738078..1738212	-	135	WP_041753625.1	entericidin A/B family lipoprotein	-
RBRH_RS18880	1738594..1738953	-	360	WP_157864390.1	hypothetical protein	-
RBRH_RS07665	1739751..1740011	+	261	WP_041753630.1	DUF1778 domain-containing protein	Antitoxin
RBRH_RS07670	1740008..1740505	+	498	WP_013435576.1	GNAT family N-acetyltransferase	Toxin
RBRH_RS07675	1740953..1741381	+	429	WP_013435578.1	membrane protein	-
RBRH_RS07680	1741541..1741828	-	288	WP_013435579.1	DUF1488 domain-containing protein	-
RBRH_RS07685	1741913..1742410	-	498	WP_041753632.1	heme-degrading domain-containing protein	-
RBRH_RS16730	1742718..1742906	-	189	WP_013435581.1	isochorismatase	-
RBRH_RS07695	1743444..1743989	+	546	WP_013435583.1	NUDIX hydrolase	-
RBRH_RS07700	1744058..1744798	+	741	WP_013435584.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1733788..1742967	9179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18143.79 Da
• Isoelectric Point: 8.3136

>T24976 WP_013435576.1 NC_014722:1740008-1740505 [Mycetohabitans rhizoxinica HKI 454]
VSVELFAPQSLCTQHRLDEFDCGESALNEWLKRRALINHLSGASHTFVAVDADQRVFGYYALAAGAVSHQQATGAVRRNM
PDPVPVMVLARLAVDLRAQGIKLGAALLQDAVCRVQAVSENVGVRALLVHALHERARQFYERYGFQSSPVHSMTLMLRLP
QGSHT

Nucleotide

• Length: 498 bp

>T24976 NC_014722:1740008-1740505 [Mycetohabitans rhizoxinica HKI 454]
GTGAGTGTGGAACTTTTTGCCCCGCAGTCCCTATGCACTCAGCATCGGCTTGATGAGTTTGACTGTGGCGAGTCAGCTTT
AAATGAGTGGCTTAAGCGTCGAGCGTTAATTAATCACCTGAGCGGGGCCAGTCATACGTTTGTCGCGGTTGATGCTGACC
AGCGCGTCTTCGGCTATTATGCGTTGGCAGCAGGGGCGGTTTCACATCAGCAAGCCACCGGCGCGGTTCGCCGCAATATG
CCAGATCCGGTTCCGGTCATGGTGCTGGCTCGGCTTGCAGTCGATCTACGGGCTCAGGGCATCAAACTCGGTGCGGCCTT
GCTGCAAGACGCAGTATGCCGAGTACAGGCCGTATCGGAGAATGTCGGCGTTCGGGCCTTGCTTGTCCATGCGCTGCATG
AGCGTGCACGTCAGTTCTACGAGCGCTACGGATTTCAATCCTCGCCAGTGCATTCTATGACGTTAATGTTGCGACTACCG
CAAGGCAGCCATACGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9749.33 Da
• Isoelectric Point: 8.0546

>AT24976 WP_041753630.1 NC_014722:1739751-1740011 [Mycetohabitans rhizoxinica HKI 454]
MRDAAINLRALPEQRDLIDHAASLLGKNRSDFMLEVACERAQAVVLDRVFFHLDADKFQQFVAMLDAPQSANPGLKRLMN
IKAPWK

Nucleotide

• Length: 261 bp

>AT24976 NC_014722:1739751-1740011 [Mycetohabitans rhizoxinica HKI 454]
ATGCGAGACGCCGCTATCAATCTACGTGCCCTGCCTGAACAGCGCGATTTGATCGACCATGCCGCAAGCTTACTAGGCAA
GAACCGCTCTGACTTCATGCTCGAAGTCGCGTGCGAGCGAGCACAAGCGGTTGTGCTGGATCGGGTGTTTTTCCATTTGG
ACGCTGACAAGTTCCAGCAGTTCGTGGCAATGCTAGACGCGCCGCAAAGCGCCAATCCGGGCCTTAAGCGCTTGATGAAC
ATTAAGGCCCCCTGGAAGTGA

Structures

Toxin

• AlphaFold DB: E5ARW5