Detailed information of TA system TA024975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1555343..1555920
• Replicon: chromosome
• Accession: NC_014722
• Organism: Burkholderia rhizoxinica HKI 454

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E5ARJ7
• Locus tag: RBRH_RS07245
• Protein ID: WP_013435458.1
• Coordinates: 1555555..1555920 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: RBRH_RS07240
• Protein ID: WP_041754296.1
• Coordinates: 1555343..1555561 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBRH_RS07225	1551350..1551553	+	204	WP_013435453.1	DUF1289 domain-containing protein	-
RBRH_RS17180	1552135..1552344	+	210	WP_013435454.1	DUF2945 domain-containing protein	-
RBRH_RS07230	1552454..1553665	+	1212	WP_013435455.1	putative DNA modification/repair radical SAM protein	-
RBRH_RS07235	1553662..1555233	+	1572	WP_013435456.1	UdgX family uracil-DNA binding protein	-
RBRH_RS07240	1555343..1555561	+	219	WP_041754296.1	ribbon-helix-helix protein, CopG family	Antitoxin
RBRH_RS07245	1555555..1555920	+	366	WP_013435458.1	type II toxin-antitoxin system VapC family toxin	Toxin
RBRH_RS17185	1556044..1556205	+	162	Protein_1441	transposase	-
RBRH_RS07250	1556414..1556653	+	240	WP_041754297.1	ribbon-helix-helix domain-containing protein	-
RBRH_RS07255	1556653..1557072	+	420	WP_013435461.1	putative toxin-antitoxin system toxin component, PIN family	-
RBRH_RS07260	1557092..1558192	-	1101	WP_157864480.1	IS5 family transposase	-
RBRH_RS18835	1558232..1558576	-	345	WP_013435463.1	transposase	-
RBRH_RS07270	1558846..1560168	+	1323	WP_013435465.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1558846..1560168	1322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13725.83 Da
• Isoelectric Point: 7.0147

>T24975 WP_013435458.1 NC_014722:1555555-1555920 [Mycetohabitans rhizoxinica HKI 454]
MVKALFDTNILIDYLSGINAARKELERYEYRAISTITWMEILVSASTEEESAIRAWLSSFDVVALDNAIANRAVEIRKTK
RIRLPDAIAWATAQVHSLLLVSRNTKDFPADEPGMRVPYKI

Nucleotide

• Length: 366 bp

>T24975 NC_014722:1555555-1555920 [Mycetohabitans rhizoxinica HKI 454]
ATGGTAAAGGCGCTTTTTGACACGAACATCCTGATCGACTATCTAAGCGGAATCAACGCTGCGAGGAAGGAGCTGGAGCG
CTATGAATATCGTGCGATCAGCACGATTACCTGGATGGAGATCCTTGTTAGTGCCTCCACAGAGGAAGAATCTGCGATTC
GAGCATGGCTCAGTTCATTCGATGTAGTCGCCCTCGATAATGCGATCGCGAACCGGGCAGTGGAGATTCGAAAAACCAAG
CGGATTCGGTTGCCGGATGCCATTGCATGGGCGACGGCACAGGTGCACTCGCTGCTGCTTGTCTCACGCAATACGAAAGA
TTTCCCTGCGGACGAACCAGGCATGCGCGTCCCGTACAAAATTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8318.59 Da
• Isoelectric Point: 7.0129

>AT24975 WP_041754296.1 NC_014722:1555343-1555561 [Mycetohabitans rhizoxinica HKI 454]
MSRILVELPDSRIEELAVIVETEHRPRAAIIRDAIEAYISQRKRTLGADVLGLWKSKKVDGLAYQQELRSEW

Nucleotide

• Length: 219 bp

>AT24975 NC_014722:1555343-1555561 [Mycetohabitans rhizoxinica HKI 454]
ATGAGTCGTATCCTCGTTGAATTACCGGATTCGCGGATTGAGGAGTTGGCGGTTATCGTGGAGACCGAACATCGTCCACG
CGCTGCCATCATTCGTGACGCAATCGAGGCGTATATCTCCCAGCGCAAGCGCACACTCGGGGCAGACGTCCTCGGGTTGT
GGAAGAGCAAGAAAGTCGACGGCCTTGCCTACCAGCAGGAGCTCCGCTCCGAATGGTAA

Structures

Toxin

• AlphaFold DB: E5ARJ7