Detailed information of TA system TA024973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 329488..330017
• Replicon: plasmid pBRH01
• Accession: NC_014718
• Organism: Burkholderia rhizoxinica HKI 454

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: E5AUF5
• Locus tag: RBRH_RS13365
• Protein ID: WP_013428590.1
• Coordinates: 329667..330017 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: RBRH_RS13360
• Protein ID: WP_013428589.1
• Coordinates: 329488..329673 (+)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBRH_RS19695	324612..324815	-	204	WP_013428580.1	hypothetical protein	-
RBRH_RS13325	324910..325110	-	201	Protein_227	tyrosine-type recombinase/integrase	-
RBRH_RS13330	325654..325982	-	329	Protein_228	transcriptional regulator	-
RBRH_RS13335	325979..326301	-	323	Protein_229	type II toxin-antitoxin system RelE/ParE family toxin	-
RBRH_RS13340	326588..327877	-	1290	Protein_230	tyrosine-type recombinase/integrase	-
RBRH_RS13345	327969..328628	+	660	WP_013428586.1	DNA-binding protein	-
RBRH_RS13350	328774..329118	-	345	WP_013428587.1	hypothetical protein	-
RBRH_RS13355	329115..329408	-	294	WP_013428588.1	hypothetical protein	-
RBRH_RS13360	329488..329673	+	186	WP_013428589.1	MazE protein	Antitoxin
RBRH_RS13365	329667..330017	+	351	WP_013428590.1	type II toxin-antitoxin system ChpB family toxin	Toxin
RBRH_RS13370	330170..330576	+	407	Protein_236	hypothetical protein	-
RBRH_RS13375	330796..331059	+	264	WP_104078549.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
RBRH_RS13380	331031..331360	+	330	WP_013428593.1	type II toxin-antitoxin system YafQ family toxin	-
RBRH_RS13385	331512..332426	-	915	Protein_239	IS110 family transposase	-
RBRH_RS13390	333213..333530	-	318	WP_013428596.1	hypothetical protein	-
RBRH_RS16455	333849..334034	-	186	WP_013428597.1	hypothetical protein	-
RBRH_RS19280	334022..334378	-	357	WP_013428598.1	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	icl / gnd / pilW	1..822304	822304
	-	flank	IS/Tn	-	-	333849..334034	185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-114)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12381.27 Da
• Isoelectric Point: 11.1777

>T24973 WP_013428590.1 NC_014718:329667-330017 [Mycetohabitans rhizoxinica HKI 454]
MVRRVKFARGDIVRVSLNSTAGREQQGDFRPALVLSAAAFNALGVALVAPITQGDASARFARFAVPLSGSGTETQGVALV
NMARMLDLEARGARKLERAPVEVVEDALARFQTIIE

Nucleotide

• Length: 351 bp

>T24973 NC_014718:329667-330017 [Mycetohabitans rhizoxinica HKI 454]
TTGGTGAGGCGGGTCAAGTTCGCGCGGGGCGATATCGTGCGCGTGAGCCTAAACTCGACTGCCGGGCGAGAGCAGCAAGG
TGATTTTCGCCCAGCGCTCGTTCTATCTGCGGCGGCGTTCAACGCCTTGGGCGTGGCGCTCGTTGCCCCGATCACCCAGG
GCGACGCCTCTGCTCGCTTTGCTAGGTTCGCCGTACCGCTTTCCGGTTCTGGAACCGAGACGCAAGGTGTAGCGCTCGTC
AACATGGCGCGTATGCTCGACCTGGAGGCGCGCGGCGCGCGTAAGCTCGAACGTGCGCCGGTTGAAGTGGTTGAGGACGC
GCTGGCTCGGTTTCAGACGATTATTGAATGA

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 6759.75 Da
• Isoelectric Point: 4.5453

>AT24973 WP_013428589.1 NC_014718:329488-329673 [Mycetohabitans rhizoxinica HKI 454]
VFLASVGSSLNADVRPDGVMLAPARRKYSLDDLIAQCDMKTFFPEDMAAWSEVKPVGREAW

Nucleotide

• Length: 186 bp

>AT24973 NC_014718:329488-329673 [Mycetohabitans rhizoxinica HKI 454]
GTGTTTCTCGCGTCGGTCGGCAGTTCGCTAAACGCCGACGTGCGTCCGGATGGCGTAATGCTGGCCCCGGCCCGCCGCAA
ATATTCGCTGGACGACCTTATTGCCCAATGCGACATGAAGACTTTTTTTCCGGAGGACATGGCGGCATGGAGTGAGGTCA
AACCGGTGGGGCGTGAGGCTTGGTGA

Structures

Toxin

• AlphaFold DB: E5AUF5