Detailed information of TA system TA024972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 323626..324263
• Replicon: plasmid pBRH01
• Accession: NC_014718
• Organism: Burkholderia rhizoxinica HKI 454

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RBRH_RS13315
• Protein ID: WP_041754720.1
• Coordinates: 323862..324263 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A2P5K7D8
• Locus tag: RBRH_RS13310
• Protein ID: WP_041754719.1
• Coordinates: 323626..323862 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBRH_RS16790	319090..319512	+	423	Protein_217	ATP-binding protein	-
RBRH_RS13285	319588..319956	+	369	Protein_218	IS5/IS1182 family transposase	-
RBRH_RS13290	320106..320537	-	432	Protein_219	IS21-like element ISBmu3 family helper ATPase IstB	-
RBRH_RS13295	320553..322102	-	1550	Protein_220	IS21 family transposase	-
RBRH_RS17780	322331..322534	+	204	Protein_221	transposase domain-containing protein	-
RBRH_RS13305	322546..323139	+	594	Protein_222	plasmid pRiA4b ORF-3 family protein	-
RBRH_RS13310	323626..323862	+	237	WP_041754719.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RBRH_RS13315	323862..324263	+	402	WP_041754720.1	type II toxin-antitoxin system VapC family toxin	Toxin
RBRH_RS13320	324386..324652	-	267	WP_157864530.1	hypothetical protein	-
RBRH_RS19695	324612..324815	-	204	WP_013428580.1	hypothetical protein	-
RBRH_RS13325	324910..325110	-	201	Protein_227	tyrosine-type recombinase/integrase	-
RBRH_RS13330	325654..325982	-	329	Protein_228	transcriptional regulator	-
RBRH_RS13335	325979..326301	-	323	Protein_229	type II toxin-antitoxin system RelE/ParE family toxin	-
RBRH_RS13340	326588..327877	-	1290	Protein_230	tyrosine-type recombinase/integrase	-
RBRH_RS13345	327969..328628	+	660	WP_013428586.1	DNA-binding protein	-
RBRH_RS13350	328774..329118	-	345	WP_013428587.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	icl / gnd / pilW	1..822304	822304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14926.22 Da
• Isoelectric Point: 6.9521

>T24972 WP_041754720.1 NC_014718:323862-324263 [Mycetohabitans rhizoxinica HKI 454]
MPRFMLDTNMCIYLMKNQPEEVAKRFAQCYVGDVVMSAVTYAELEYGVVVSANRTRERRNLAALIEDIPVAPFDTAAATA
YGPVRKATSERKKDALDKLIAAHAIALDVILVTNNERDFASYPGIRLENWLNK

Nucleotide

• Length: 402 bp

>T24972 NC_014718:323862-324263 [Mycetohabitans rhizoxinica HKI 454]
ATGCCGCGCTTTATGCTCGACACAAACATGTGCATCTACCTGATGAAGAATCAGCCTGAAGAAGTCGCGAAGCGATTTGC
GCAGTGCTACGTCGGGGATGTGGTTATGTCCGCAGTCACTTACGCTGAACTTGAATACGGCGTCGTAGTGTCTGCGAACC
GAACTCGCGAGCGCCGCAATCTCGCCGCCCTAATCGAAGATATTCCTGTGGCCCCGTTCGACACTGCCGCAGCAACGGCC
TACGGGCCTGTGCGCAAAGCGACGAGCGAGCGCAAGAAGGATGCTCTTGACAAGCTGATCGCCGCGCACGCGATTGCCCT
GGACGTTATTCTCGTGACCAACAACGAGCGCGATTTTGCCAGCTATCCCGGTATCAGGCTCGAAAACTGGCTCAACAAGT
AG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8931.14 Da
• Isoelectric Point: 7.0161

>AT24972 WP_041754719.1 NC_014718:323626-323862 [Mycetohabitans rhizoxinica HKI 454]
MHTTRVFKNGNSQAVRIPADLAYDRSDVEIEIERVGDEIRIRPMRRPLTGVLKKFAKFGPDFMAEGRGEQEQAEREGL

Nucleotide

• Length: 237 bp

>AT24972 NC_014718:323626-323862 [Mycetohabitans rhizoxinica HKI 454]
ATGCATACCACCCGCGTTTTCAAGAATGGCAACTCGCAAGCCGTTCGCATTCCCGCCGACCTTGCTTACGACCGGAGCGA
TGTCGAAATCGAAATTGAGCGCGTAGGCGATGAGATCCGTATCCGTCCGATGCGTCGTCCGCTGACTGGCGTGCTCAAGA
AGTTCGCGAAATTCGGGCCGGATTTCATGGCTGAAGGCCGCGGCGAGCAGGAGCAGGCGGAGCGCGAGGGATTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2P5K7D8