Detailed information of TA system TA024971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-RelB
• Location: 307268..307794
• Replicon: plasmid pBRH01
• Accession: NC_014718
• Organism: Burkholderia rhizoxinica HKI 454

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E5AUC7
• Locus tag: RBRH_RS13255
• Protein ID: WP_013428562.1
• Coordinates: 307525..307794 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RBRH_RS13250
• Protein ID: WP_041754717.1
• Coordinates: 307268..307528 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RBRH_RS13215	302862..303251	+	390	WP_013428552.1	DUF488 family protein	-
RBRH_RS19255	303557..303814	-	258	WP_157864529.1	hypothetical protein	-
RBRH_RS13225	303816..304136	+	321	WP_041754716.1	DUF4148 domain-containing protein	-
RBRH_RS13230	304478..304945	+	468	Protein_208	GNAT family N-acetyltransferase	-
RBRH_RS13235	305021..305896	-	876	WP_013428558.1	helix-turn-helix transcriptional regulator	-
RBRH_RS13240	306198..306542	+	345	Protein_210	S-adenosylmethionine:2-demethylmenaquinone methyltransferase	-
RBRH_RS13245	306558..307022	+	465	WP_013428560.1	gamma-glutamylcyclotransferase	-
RBRH_RS13250	307268..307528	+	261	WP_041754717.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
RBRH_RS13255	307525..307794	+	270	WP_013428562.1	Txe/YoeB family addiction module toxin	Toxin
RBRH_RS19260	307949..308355	-	407	Protein_214	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	icl / gnd / pilW	1..822304	822304
	-	flank	IS/Tn	-	-	308023..308364	341

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10608.90 Da
• Isoelectric Point: 7.9861

>T24971 WP_013428562.1 NC_014718:307525-307794 [Mycetohabitans rhizoxinica HKI 454]
VSDRRVLFTPDAWEDYVYWQGQDRKTLKRINQLIREAQRAPFEGIGKPEPLKANLSGFWSRRIDDTNRLVYEADDFQISV
ISCRYHYQA

Nucleotide

• Length: 270 bp

>T24971 NC_014718:307525-307794 [Mycetohabitans rhizoxinica HKI 454]
GTGAGCGATCGCCGCGTGCTTTTTACGCCCGACGCATGGGAGGACTACGTCTACTGGCAAGGACAGGACCGCAAGACTCT
CAAACGCATCAACCAGCTCATTCGCGAAGCACAGCGTGCGCCGTTCGAGGGGATCGGTAAGCCGGAGCCACTGAAGGCAA
ACCTCTCCGGCTTCTGGTCCCGCCGCATCGACGACACGAACCGCCTCGTGTATGAGGCAGACGATTTCCAGATCAGCGTC
ATCTCCTGCCGCTACCACTACCAGGCGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9694.94 Da
• Isoelectric Point: 6.2201

>AT24971 WP_041754717.1 NC_014718:307268-307528 [Mycetohabitans rhizoxinica HKI 454]
MRTIHFSDARSNLKTVIDQVVDDADVTLVTRRDAPNAVIMSQDYYDSLMETVHLLRSPANVGHLERSIAQLRKGKATERK
LAEDAE

Nucleotide

• Length: 261 bp

>AT24971 NC_014718:307268-307528 [Mycetohabitans rhizoxinica HKI 454]
ATGCGTACAATTCATTTTTCGGATGCACGCAGTAACCTCAAAACCGTGATCGACCAGGTTGTCGACGATGCCGACGTGAC
GCTCGTCACGCGTCGCGATGCGCCCAATGCTGTCATCATGTCGCAGGATTATTACGACAGCCTGATGGAGACAGTACACC
TGCTGCGCTCGCCGGCCAATGTCGGCCACCTCGAGCGCTCGATCGCGCAGCTGCGCAAGGGCAAAGCAACGGAGCGCAAG
CTGGCCGAGGATGCTGAGTGA

Structures

Toxin

• AlphaFold DB: E5AUC7