Detailed information of TA system TA024966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2702651..2703282
• Replicon: chromosome
• Accession: NC_014664
• Organism: Rhodomicrobium vannielii ATCC 17100

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RVAN_RS12495
• Protein ID: WP_013420073.1
• Coordinates: 2702651..2703049 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E3I5H5
• Locus tag: RVAN_RS12500
• Protein ID: WP_013420074.1
• Coordinates: 2703049..2703282 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVAN_RS12475	2697931..2698218	+	288	WP_155942552.1	IS66 family insertion sequence element accessory protein TnpB	-
RVAN_RS12480	2698278..2699882	+	1605	WP_013420070.1	IS66 family transposase	-
RVAN_RS12485	2700233..2700409	+	177	WP_013420071.1	IS66 family insertion sequence element accessory protein TnpB	-
RVAN_RS12490	2700729..2702228	-	1500	WP_155942569.1	phosphomannomutase	-
RVAN_RS12495	2702651..2703049	-	399	WP_013420073.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RVAN_RS12500	2703049..2703282	-	234	WP_013420074.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RVAN_RS12505	2703360..2705216	-	1857	WP_013420075.1	relaxase/mobilization nuclease domain-containing protein	-
RVAN_RS12510	2705213..2705491	-	279	WP_013420076.1	hypothetical protein	-
RVAN_RS12515	2705746..2707530	-	1785	WP_013420077.1	DUF927 domain-containing protein	-
RVAN_RS12520	2707586..2707834	-	249	WP_013420078.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14681.89 Da
• Isoelectric Point: 7.4016

>T24966 WP_013420073.1 NC_014664:c2703049-2702651 [Rhodomicrobium vannielii ATCC 17100]
MLGYMLDTNICIYVMKTFPPAVRDKFNALAEHLCISSITLGELHYGAQKSARRDENLTAIEHFVARLDVLPFGNKAAAHY
GQVRAELERAGTPCGPHDMQIGGHARSEGLIVVTNNMREFARMPGVRAENWL

Nucleotide

• Length: 399 bp

>T24966 NC_014664:c2703049-2702651 [Rhodomicrobium vannielii ATCC 17100]
ATGCTCGGCTACATGCTCGATACAAATATCTGCATCTATGTGATGAAGACCTTCCCGCCTGCCGTCCGGGACAAATTCAA
TGCGCTGGCTGAACATCTTTGCATATCGAGCATCACGCTTGGCGAATTGCATTACGGTGCGCAGAAGTCCGCCCGCCGCG
ACGAAAACCTGACCGCGATCGAACACTTCGTGGCGCGGCTGGACGTGCTGCCGTTCGGCAATAAAGCCGCCGCTCATTAT
GGGCAGGTCCGGGCTGAACTGGAGCGAGCGGGAACACCTTGCGGCCCCCACGATATGCAGATCGGCGGTCATGCCCGCAG
CGAAGGATTGATTGTCGTGACGAATAACATGCGCGAGTTTGCCAGGATGCCCGGCGTCAGGGCCGAGAATTGGCTGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8840.05 Da
• Isoelectric Point: 7.2051

>AT24966 WP_013420074.1 NC_014664:c2703282-2703049 [Rhodomicrobium vannielii ATCC 17100]
MTSSTVFTSNRSQAVRLPKAVAFPEDVHHVDILKIGRSRLIVPQGKRWDDLFLSGPRVSEDFMVEREQPKAEEREAF

Nucleotide

• Length: 234 bp

>AT24966 NC_014664:c2703282-2703049 [Rhodomicrobium vannielii ATCC 17100]
ATGACCAGTTCGACCGTCTTCACAAGCAACCGCAGCCAGGCTGTTCGCCTCCCAAAGGCTGTCGCTTTTCCCGAGGACGT
GCACCATGTCGATATTCTTAAGATCGGGCGCAGCCGCCTGATCGTACCGCAGGGCAAGAGATGGGACGATCTTTTCCTGA
GCGGACCGCGTGTCAGCGAGGATTTCATGGTCGAGCGCGAGCAGCCGAAGGCGGAAGAGCGCGAGGCATTCTGA

Structures

Antitoxin

• AlphaFold DB: E3I5H5