Detailed information of TA system TA024965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 1965818..1967342
• Replicon: chromosome
• Accession: NC_014664
• Organism: Rhodomicrobium vannielii ATCC 17100

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: E3HZK6
• Locus tag: RVAN_RS09045
• Protein ID: WP_013419431.1
• Coordinates: 1966467..1967342 (+)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: RVAN_RS09040
• Protein ID: WP_041788757.1
• Coordinates: 1965818..1966474 (+)
• Length: 219 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVAN_RS09020	1962968..1963285	-	318	WP_013419425.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
RVAN_RS18940	1963368..1963967	+	600	WP_155942405.1	helix-turn-helix transcriptional regulator	-
RVAN_RS09030	1964070..1964468	+	399	WP_013419427.1	hypothetical protein	-
RVAN_RS09035	1965457..1965696	+	240	WP_081449565.1	pyocin activator PrtN family protein	-
RVAN_RS09040	1965818..1966474	+	657	WP_041788757.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
RVAN_RS09045	1966467..1967342	+	876	WP_013419431.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
RVAN_RS09055	1968032..1968784	-	753	WP_013419433.1	porin family protein	-
RVAN_RS09060	1968982..1971648	-	2667	WP_013419434.1	spermidine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1954837..1967802	12965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-257)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (19-61)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 32626.60 Da
• Isoelectric Point: 5.2334

>T24965 WP_013419431.1 NC_014664:1966467-1967342 [Rhodomicrobium vannielii ATCC 17100]
MPNIAASVRARLRNVAATRNEPVELLLTRYVLERLLYRLSTTEHRKRFVLKGAMLMRMWFDMPFRPTRDLDLLGFGDPDP
EAMLAIFRGICSIQLDDGVEFDAEGLRIHRIREDNEYGGLRLKTIATLAGARIAVTIDVGFGDAVEPEITDVELPVLLDL
PPPRLYAYPRETVIAEKFQAMVNLGRANSRMKDFYDIWQLSIAYDFHGESLPRAIAATFARRNTPVPSESPDALTAAFAE
DLVKQQQWNSFLQDIDAPFVPLAEILAALNSFLMPHAEAARTIAASKGLPS

Nucleotide

• Length: 876 bp

>T24965 NC_014664:1966467-1967342 [Rhodomicrobium vannielii ATCC 17100]
ATGCCTAACATTGCTGCCTCTGTTCGCGCTCGCCTTCGTAACGTCGCGGCCACGCGCAATGAACCCGTCGAATTGCTGTT
GACGCGGTATGTGTTGGAGCGCCTTCTCTACCGGCTCAGCACCACCGAACATCGCAAGCGCTTCGTCCTAAAAGGCGCAA
TGCTGATGAGGATGTGGTTCGACATGCCATTTCGGCCAACGCGCGACTTGGATCTTCTTGGCTTTGGTGATCCAGATCCA
GAAGCGATGCTCGCTATTTTCAGAGGCATATGCTCGATTCAGCTTGATGATGGCGTCGAATTCGACGCTGAGGGTTTACG
CATCCACCGCATCCGCGAGGACAACGAGTATGGGGGTCTGCGGCTCAAAACAATCGCGACGCTTGCAGGAGCGCGGATTG
CCGTTACCATCGACGTCGGTTTTGGTGATGCCGTCGAACCTGAAATCACCGATGTCGAACTGCCTGTGCTACTCGACCTG
CCGCCTCCACGTCTTTACGCCTATCCGCGAGAAACGGTGATCGCCGAAAAGTTTCAGGCGATGGTCAATCTCGGCCGCGC
CAACAGCCGCATGAAGGACTTTTATGATATTTGGCAGCTTTCAATTGCTTACGACTTTCATGGCGAAAGCCTGCCGCGAG
CCATCGCTGCAACATTTGCACGGCGCAACACGCCAGTTCCTTCGGAAAGCCCCGACGCGTTGACGGCGGCGTTCGCCGAG
GACCTTGTCAAACAACAACAATGGAACTCGTTTTTGCAGGACATCGACGCGCCGTTTGTGCCTCTCGCAGAAATCCTTGC
AGCTTTGAACTCTTTTCTTATGCCTCATGCGGAAGCCGCTCGGACGATAGCGGCTTCAAAGGGGCTGCCTTCCTAA

Antitoxin

Protein

• Length: 219 a.a.
• Molecular weight: 24283.32 Da
• Isoelectric Point: 10.6833

>AT24965 WP_041788757.1 NC_014664:1965818-1966474 [Rhodomicrobium vannielii ATCC 17100]
MLAAVNTRYPTQQDRAIALLAERGMAKLSEFTASGITAATISRMKERGLILQLSRGLYQLPDAPLDGNHSLAEAAKRVPK
GVICLVSALAYHELTDTIPRLVWIAIGARQKKPVVKTPPLNIVRFGQMVFSTGIEEHMIEGVRVRIYSPAKTVVDLFRYR
QREGRRYKSSPGINPVLEGMREALRQRKATPADIARYAMEAGVWSNLRPYLEAMTSHA

Nucleotide

• Length: 657 bp

>AT24965 NC_014664:1965818-1966474 [Rhodomicrobium vannielii ATCC 17100]
ATGCTCGCGGCTGTGAACACTCGCTATCCAACTCAACAAGATCGCGCCATTGCTCTCCTCGCCGAGCGGGGTATGGCCAA
GCTTTCCGAGTTTACGGCAAGCGGCATCACTGCGGCCACCATATCCCGCATGAAAGAACGCGGCCTTATCCTTCAGCTCA
GCCGTGGCCTTTACCAACTCCCCGATGCTCCTCTTGACGGAAACCACTCTCTTGCGGAAGCAGCGAAGCGCGTGCCTAAA
GGAGTGATTTGCCTCGTGTCGGCGCTTGCCTATCATGAGTTGACCGATACGATACCGCGCCTCGTTTGGATCGCAATCGG
CGCTAGGCAGAAGAAGCCCGTGGTCAAAACGCCGCCGCTTAATATCGTGCGCTTTGGACAGATGGTCTTCTCCACCGGCA
TCGAGGAACACATGATCGAAGGCGTTAGAGTGCGCATCTATAGCCCCGCCAAAACCGTCGTTGATCTTTTCCGTTACCGC
CAGCGCGAAGGCAGACGCTATAAGAGTAGTCCAGGCATCAACCCCGTGCTGGAAGGCATGCGCGAAGCATTGCGGCAGCG
GAAGGCGACGCCGGCCGATATCGCACGCTACGCCATGGAAGCAGGGGTTTGGAGCAATCTGCGCCCTTATCTTGAAGCGA
TGACTTCACATGCCTAA

Structures

Toxin

• AlphaFold DB: E3HZK6