Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 40419..41217 Replicon plasmid plas1
Accession NZ_CP099878
Organism Escherichia albertii strain 247_2_EW_B

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag NIZ22_RS23095 Protein ID WP_000072677.1
Coordinates 40419..40940 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag NIZ22_RS23100 Protein ID WP_001351987.1
Coordinates 40948..41217 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIZ22_RS23080 (NIZ22_23060) 38968..39291 + 324 WP_000064175.1 hypothetical protein -
NIZ22_RS23085 (NIZ22_23065) 39305..39997 + 693 WP_012640731.1 hypothetical protein -
NIZ22_RS23090 (NIZ22_23070) 39999..40250 + 252 WP_000901559.1 hypothetical protein -
NIZ22_RS23095 (NIZ22_23075) 40419..40940 - 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
NIZ22_RS23100 (NIZ22_23080) 40948..41217 - 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
NIZ22_RS23105 (NIZ22_23085) 41536..42204 + 669 WP_000161228.1 division plane positioning ATPase MipZ -
NIZ22_RS23110 (NIZ22_23090) 42210..42563 + 354 WP_160378290.1 hypothetical protein -
NIZ22_RS23115 (NIZ22_23095) 42616..43383 - 768 WP_000342417.1 hypothetical protein -
NIZ22_RS23120 (NIZ22_23100) 43666..44406 + 741 WP_187192596.1 hypothetical protein -
NIZ22_RS23125 (NIZ22_23105) 44450..45790 + 1341 WP_000137333.1 DnaB-like helicase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..123684 123684


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T249634 WP_000072677.1 NZ_CP099878:c40940-40419 [Escherichia albertii]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT249634 WP_001351987.1 NZ_CP099878:c41217-40948 [Escherichia albertii]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References