Detailed information of TA system TA024963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-HTH_XRE
• Location: 1401984..1403284
• Replicon: chromosome
• Accession: NC_014644
• Organism: Gardnerella vaginalis ATCC 14019

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: HMPREF0421_RS05645
• Protein ID: WP_004113001.1
• Coordinates: 1401984..1402793 (-)
• Length: 270 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: S4HGB9
• Locus tag: HMPREF0421_RS05650
• Protein ID: WP_004113003.1
• Coordinates: 1402811..1403284 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMPREF0421_RS05625	1397889..1398626	+	738	WP_004112993.1	thiamine diphosphokinase	-
HMPREF0421_RS05630	1398802..1399857	+	1056	WP_004112995.1	type I glyceraldehyde-3-phosphate dehydrogenase	-
HMPREF0421_RS05635	1399968..1400468	+	501	WP_004112997.1	Cys-tRNA(Pro) deacylase	-
HMPREF0421_RS05640	1400516..1401475	+	960	WP_004112999.1	aldose 1-epimerase family protein	-
HMPREF0421_RS05645	1401984..1402793	-	810	WP_004113001.1	zeta toxin family protein	Toxin
HMPREF0421_RS05650	1402811..1403284	-	474	WP_004113003.1	helix-turn-helix domain-containing protein	Antitoxin
HMPREF0421_RS05655	1403822..1405258	-	1437	WP_009994216.1	hypothetical protein	-
HMPREF0421_RS05660	1406115..1407125	+	1011	WP_004116755.1	UDP-glucose 4-epimerase GalE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (34-221)

Antitoxin

• Cro/C1-type helix-turn-helix domain (6-59)

Sequences

Toxin

Protein

• Length: 270 a.a.
• Molecular weight: 30905.69 Da
• Isoelectric Point: 8.9574

>T24963 WP_004113001.1 NC_014644:c1402793-1401984 [Gardnerella vaginalis ATCC 14019]
MGKCDFAMVIEDFSEEYFTHCLLRNLRSLQMYKHADLHPKAVLLGGQSGAGKTTIHVIKQKEFNGNIIILDADSYRTQHP
YYCELLDKYGKESVKYTSAFSAKMVRSLIDELSSKKYNLIIEGTLRSVEVPSSTAKMLIDRGYRISLAVIATKPELSWIS
TLIRYREMYLEDPKSARATPKDHHDNIVNNIVDNLLKLEQSKIFDRIQIYKRDEKCIYDSDSYKNSPNITAASVLKEVLF
GKKTIDEKKLICHAKNRLNELDKLIDKSL

Nucleotide

• Length: 810 bp

>T24963 NC_014644:c1402793-1401984 [Gardnerella vaginalis ATCC 14019]
ATGGGTAAGTGTGATTTTGCTATGGTTATAGAAGATTTTAGCGAAGAGTATTTTACTCACTGTTTGTTAAGAAATTTACG
TTCTTTACAAATGTATAAGCATGCAGATTTGCATCCTAAGGCGGTTTTACTCGGTGGTCAAAGTGGTGCTGGCAAAACGA
CCATTCATGTAATAAAACAAAAAGAATTTAACGGTAACATAATAATCCTTGATGCTGATAGCTATAGGACTCAACACCCT
TATTATTGCGAGTTGCTTGATAAATACGGTAAAGAAAGTGTTAAATATACGAGTGCTTTTTCAGCAAAAATGGTGAGAAG
CTTAATTGATGAGTTAAGTTCTAAAAAATATAATCTCATAATTGAAGGTACTTTGCGCAGTGTTGAAGTTCCTAGTAGTA
CTGCGAAAATGTTGATAGATCGTGGTTACCGTATTTCTCTTGCTGTCATTGCAACAAAACCTGAATTGTCATGGATCAGT
ACGCTGATTCGCTACAGGGAAATGTATTTGGAAGATCCTAAAAGTGCTAGGGCAACTCCTAAGGATCATCACGACAATAT
TGTGAACAATATAGTAGATAACTTGTTGAAATTAGAACAATCGAAAATATTTGACAGAATTCAGATTTACAAGCGAGACG
AAAAATGTATATATGATTCTGATTCGTATAAAAATAGTCCAAATATTACAGCAGCTAGTGTTCTTAAGGAAGTGCTCTTT
GGCAAGAAAACTATTGATGAGAAAAAGTTGATTTGCCACGCTAAGAATAGGTTGAATGAACTAGACAAGTTGATAGATAA
AAGTTTGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 18016.48 Da
• Isoelectric Point: 5.3394

>AT24963 WP_004113003.1 NC_014644:c1403284-1402811 [Gardnerella vaginalis ATCC 14019]
MVSVNIRALRRSKKITQQELAEALGVSRNSIVRYENGTSAISTRLIDKICDKFKVSLFDIVHEKELSASDKYDLNMKIEV
LKERGALLLARLYKYEDSMNIQFDDSSNAWIAISDDLSDVINTKIYTSTNFEDINRYIGYLDGIERLLEYVSCNKNS

Nucleotide

• Length: 474 bp

>AT24963 NC_014644:c1403284-1402811 [Gardnerella vaginalis ATCC 14019]
ATGGTTTCTGTGAATATTCGAGCACTACGTCGCTCAAAAAAAATAACTCAACAAGAGTTAGCTGAAGCATTAGGAGTATC
TCGTAATAGCATTGTGCGATACGAAAATGGTACAAGTGCTATTTCAACTAGGTTAATAGATAAGATTTGTGATAAATTCA
AGGTTTCTCTTTTTGATATCGTACATGAAAAAGAATTATCTGCATCTGATAAATACGATTTGAATATGAAGATTGAAGTT
TTAAAGGAGCGTGGCGCACTTTTACTGGCTCGTTTATATAAATACGAGGATAGTATGAATATACAATTTGATGATTCTTC
TAATGCATGGATTGCAATTAGTGATGATTTATCAGATGTAATTAATACAAAGATTTATACATCTACAAATTTTGAAGATA
TAAATCGTTATATCGGATATTTAGATGGCATAGAACGCTTGCTTGAATACGTAAGTTGCAATAAAAATAGTTAA

Structures

Antitoxin

• AlphaFold DB: S4HGB9