Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 79077..79608 Replicon plasmid pA81
Accession NC_014641
Organism Achromobacter xylosoxidans A8

Toxin (Protein)


Gene name relE Uniprot ID I2ALI9
Locus tag AXYL_RS32745 Protein ID WP_011114071.1
Coordinates 79327..79608 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID I2ALI8
Locus tag AXYL_RS32740 Protein ID WP_011114070.1
Coordinates 79077..79340 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXYL_RS32695 74187..74951 - 765 WP_013397098.1 ParA family protein -
AXYL_RS32700 74948..75256 - 309 WP_011255197.1 transcriptional regulator -
AXYL_RS32705 75500..75787 - 288 Protein_81 DUF2761 domain-containing protein -
AXYL_RS32710 75789..76115 - 327 WP_012478184.1 hypothetical protein -
AXYL_RS32715 76325..76561 - 237 WP_011255200.1 stable inheritance protein KleA -
AXYL_RS32720 76719..76976 - 258 WP_012478185.1 hypothetical protein -
AXYL_RS32725 76993..78201 - 1209 WP_012478186.1 hypothetical protein -
AXYL_RS32730 78213..78476 - 264 WP_011296181.1 hypothetical protein -
AXYL_RS32735 78476..78904 - 429 WP_011114069.1 antirestriction protein -
AXYL_RS32740 79077..79340 + 264 WP_011114070.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
AXYL_RS32745 79327..79608 + 282 WP_011114071.1 type II toxin-antitoxin system YafQ family toxin Toxin
AXYL_RS35115 81108..81335 - 228 WP_158307678.1 antitoxin VbhA family protein -
AXYL_RS32755 81332..81523 - 192 WP_013397101.1 hypothetical protein -
AXYL_RS32760 81643..82014 - 372 WP_013397102.1 hypothetical protein -
AXYL_RS32765 82044..82346 + 303 WP_013397103.1 hypothetical protein -
AXYL_RS32770 82384..83433 - 1050 WP_013397104.1 DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..98156 98156


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-83)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10743.19 Da        Isoelectric Point: 7.4750

>T24960 WP_011114071.1 NC_014641:79327-79608 [Achromobacter xylosoxidans A8]
MRQIERTGQFKRDYKREAKGQHRATLDADLVPVLVALADDQPLEPRHRDHALTGDWKDHRDCHVKPDLVLIYQKPDADTL
RLVRLGSHSELGL

Download         Length: 282 bp

>T24960 NC_014641:79327-79608 [Achromobacter xylosoxidans A8]
ATGCGGCAGATTGAGCGCACCGGCCAGTTCAAGCGCGACTACAAGCGCGAGGCGAAAGGGCAGCACCGGGCCACGCTCGA
CGCCGACCTGGTGCCCGTCCTGGTCGCGCTCGCCGACGACCAGCCGCTGGAACCTCGGCACCGCGACCACGCGCTGACCG
GGGACTGGAAGGATCACCGGGATTGCCACGTCAAGCCCGACCTGGTGCTGATCTACCAGAAGCCCGACGCCGACACGCTG
CGCCTGGTGCGCCTCGGCTCTCACTCCGAACTCGGCTTGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9255.82 Da        Isoelectric Point: 5.7905

>AT24960 WP_011114070.1 NC_014641:79077-79340 [Achromobacter xylosoxidans A8]
MAANQLVQTRIDGAIKEEAAAVLAAMGLTVSDAVRLLLTKVAQDKALPFEPLIPNATTIEAMKEARKGKLPRFATVNDLM
ADLHAAD

Download         Length: 264 bp

>AT24960 NC_014641:79077-79340 [Achromobacter xylosoxidans A8]
ATGGCTGCAAACCAGCTCGTACAAACGCGCATCGACGGCGCGATCAAGGAAGAAGCGGCGGCCGTCCTGGCCGCGATGGG
CCTTACCGTGTCCGACGCGGTGCGGCTGCTGCTCACGAAGGTGGCCCAGGACAAGGCGCTGCCCTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCCCGCAAGGGCAAGCTGCCGCGCTTCGCCACCGTCAACGATCTGATG
GCCGATCTGCATGCGGCAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1U9V4I5

Antitoxin

Source ID Structure
AlphaFold DB A0A1U9V3I2

References