Detailed information of TA system TA024952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1192472..1193007
• Replicon: chromosome
• Accession: NC_014625
• Organism: Ketogulonicigenium vulgare Y25

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: EIO_RS06125
• Protein ID: WP_013384044.1
• Coordinates: 1192759..1193007 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: F9Y4N2
• Locus tag: EIO_RS06120
• Protein ID: WP_013384043.1
• Coordinates: 1192472..1192762 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIO_RS06080	1187608..1188012	+	405	WP_013384036.1	hypothetical protein	-
EIO_RS06085	1188009..1189163	+	1155	WP_013384037.1	hypothetical protein	-
EIO_RS06090	1189163..1189426	+	264	WP_013384038.1	hypothetical protein	-
EIO_RS06095	1189539..1190168	+	630	WP_044008023.1	TetR/AcrR family transcriptional regulator	-
EIO_RS06100	1190168..1190503	+	336	WP_014537641.1	DoxX family protein	-
EIO_RS06105	1190651..1190914	+	264	WP_013384040.1	hypothetical protein	-
EIO_RS06110	1190914..1192218	+	1305	WP_013384041.1	hypothetical protein	-
EIO_RS06115	1192233..1192460	+	228	WP_013384042.1	hypothetical protein	-
EIO_RS06120	1192472..1192762	-	291	WP_013384043.1	BrnA antitoxin family protein	Antitoxin
EIO_RS06125	1192759..1193007	-	249	WP_013384044.1	BrnT family toxin	Toxin
EIO_RS06130	1193104..1193646	+	543	WP_013384045.1	lysozyme	-
EIO_RS06135	1193650..1193871	+	222	WP_013384046.1	hypothetical protein	-
EIO_RS17420	1193871..1194086	+	216	WP_162467560.1	hypothetical protein	-
EIO_RS06145	1194277..1194735	-	459	WP_014537644.1	sigma-70 family RNA polymerase sigma factor	-
EIO_RS06150	1195036..1195998	-	963	WP_044008024.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1153320..1194222	40902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-94)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9028.54 Da
• Isoelectric Point: 7.3199

>T24952 WP_013384044.1 NC_014625:c1193007-1192759 [Ketogulonicigenium vulgare Y25]
MMMIVWDEPKRESNLEKHGLDFADLSEEFFLSSVVVPAKNGRHMAIGKLADGTIAVVFATLGTQGLSVISMRHASKKERA
IL

Nucleotide

• Length: 249 bp

>T24952 NC_014625:c1193007-1192759 [Ketogulonicigenium vulgare Y25]
ATGATGATGATTGTCTGGGATGAACCGAAACGCGAAAGCAACCTCGAGAAACACGGGTTAGATTTCGCAGATTTGTCCGA
GGAATTCTTTCTGTCCTCGGTCGTTGTCCCGGCAAAGAACGGGCGTCACATGGCGATAGGGAAGCTTGCCGACGGAACGA
TTGCAGTGGTCTTTGCCACACTTGGAACGCAAGGCCTGTCGGTGATTTCGATGCGCCACGCCAGCAAAAAGGAAAGGGCG
ATCCTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10567.07 Da
• Isoelectric Point: 10.4185

>AT24952 WP_013384043.1 NC_014625:c1192762-1192472 [Ketogulonicigenium vulgare Y25]
MKANKLTEKEIQRRIASDPDAPEATDDQLAKAKPFAEAMPQLAENLRRNLGGRPKSDNPKVAVSLRLDQDVIEKFKAAGP
GWQSRMNKALREAAGV

Nucleotide

• Length: 291 bp

>AT24952 NC_014625:c1192762-1192472 [Ketogulonicigenium vulgare Y25]
ATGAAAGCCAACAAGCTAACCGAGAAAGAAATCCAACGCCGGATCGCAAGCGACCCGGACGCACCCGAGGCAACAGATGA
CCAGCTGGCGAAGGCGAAGCCCTTCGCCGAGGCCATGCCCCAACTGGCAGAGAACCTGCGCCGCAACCTTGGCGGCCGCC
CGAAATCCGACAACCCCAAAGTCGCGGTTTCGCTGCGCCTCGATCAAGACGTGATTGAGAAGTTCAAGGCCGCTGGCCCC
GGCTGGCAGTCGCGCATGAACAAAGCCCTGCGCGAAGCTGCGGGGGTATGA

Structures

Antitoxin

• AlphaFold DB: F9Y4N2