Detailed information of TA system TA024951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1459033..1459641
• Replicon: chromosome
• Accession: NC_014622
• Organism: Paenibacillus polymyxa SC2

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: PPSC2_RS35040
• Protein ID: WP_007429335.1
• Coordinates: 1459291..1459641 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PPSC2_RS35035
• Protein ID: WP_170970806.1
• Coordinates: 1459033..1459284 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPSC2_RS35020	1455224..1455856	+	633	WP_013369921.1	LysE family transporter	-
PPSC2_RS35025	1455967..1457244	+	1278	WP_013369922.1	outer membrane lipoprotein carrier protein LolA	-
PPSC2_RS35030	1457513..1458709	+	1197	WP_013369924.1	alanine racemase	-
PPSC2_RS35035	1459033..1459284	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
PPSC2_RS35040	1459291..1459641	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PPSC2_RS35045	1459820..1462042	+	2223	WP_187298942.1	RNA-binding transcriptional accessory protein	-
PPSC2_RS58950	1462147..1462281	-	135	WP_013369928.1	cortex morphogenetic protein CmpA	-
PPSC2_RS35050	1462425..1462937	+	513	WP_013369929.1	SprT family protein	-
PPSC2_RS35065	1463270..1464145	-	876	WP_013369930.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T24951 WP_007429335.1 NC_014622:1459291-1459641 [Paenibacillus polymyxa SC2]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T24951 NC_014622:1459291-1459641 [Paenibacillus polymyxa SC2]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACTGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACAATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGACTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT24951 WP_170970806.1 NC_014622:1459033-1459284 [Paenibacillus polymyxa SC2]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT24951 NC_014622:1459033-1459284 [Paenibacillus polymyxa SC2]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATTAACCTGACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1