Detailed information of TA system TA024950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4725922..4726538
• Replicon: chromosome
• Accession: NC_014618
• Organism: Enterobacter lignolyticus SCF1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ENTCL_RS21930
• Protein ID: WP_013368310.1
• Coordinates: 4725922..4726293 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E3G5J9
• Locus tag: ENTCL_RS21935
• Protein ID: WP_013368311.1
• Coordinates: 4726296..4726538 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENTCL_RS21915	4723430..4724323	+	894	WP_013368307.1	formate dehydrogenase subunit beta	-
ENTCL_RS21920	4724320..4724955	+	636	WP_013368308.1	formate dehydrogenase cytochrome b556 subunit	-
ENTCL_RS21925	4724952..4725881	+	930	WP_013368309.1	formate dehydrogenase accessory protein FdhE	-
ENTCL_RS21930	4725922..4726293	-	372	WP_013368310.1	type II toxin-antitoxin system VapC family toxin	Toxin
ENTCL_RS21935	4726296..4726538	-	243	WP_013368311.1	ribbon-helix-helix protein, CopG family	Antitoxin
ENTCL_RS21940	4726666..4727607	-	942	WP_013368312.1	fatty acid biosynthesis protein FabY	-
ENTCL_RS21945	4727658..4728095	-	438	WP_013368313.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ENTCL_RS21950	4728092..4728964	-	873	WP_013368314.1	virulence factor BrkB family protein	-
ENTCL_RS21955	4728958..4729557	-	600	WP_013368315.1	glucose-1-phosphatase	-
ENTCL_RS23365	4730045..4730680	+	636	WP_077264284.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13547.69 Da
• Isoelectric Point: 6.7179

>T24950 WP_013368310.1 NC_014618:c4726293-4725922 [[Enterobacter] lignolyticus SCF1]
MEHMAVFDTNILIDLFNNRIEAADAIDSTATHRAISLITWMEVLVGARKYGQEAKTAAVMSAFEIIDVSREIAERSVLLR
VKHGMKLPDAIILATAQSKNCQLISRNTKDFAGISGVVSPYQL

Nucleotide

• Length: 372 bp

>T24950 NC_014618:c4726293-4725922 [[Enterobacter] lignolyticus SCF1]
ATGGAGCATATGGCAGTATTTGATACCAATATTCTCATCGACCTTTTCAACAATCGCATAGAGGCTGCGGATGCCATTGA
TAGCACAGCGACACATCGGGCAATAAGCCTCATCACCTGGATGGAAGTGTTGGTTGGCGCTCGTAAGTACGGTCAGGAAG
CCAAAACGGCGGCCGTAATGAGCGCGTTTGAGATTATCGATGTTTCGCGTGAGATTGCAGAACGCAGTGTATTACTTCGT
GTAAAACACGGGATGAAGCTTCCGGATGCCATCATTCTGGCTACCGCGCAAAGTAAAAACTGCCAACTTATCTCTCGTAA
TACGAAAGATTTTGCGGGTATCTCAGGGGTAGTTTCACCCTATCAGCTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9494.68 Da
• Isoelectric Point: 4.3324

>AT24950 WP_013368311.1 NC_014618:c4726538-4726296 [[Enterobacter] lignolyticus SCF1]
MSHVVDLDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQYLDKQSSTVIREALGLWGNQEEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT24950 NC_014618:c4726538-4726296 [[Enterobacter] lignolyticus SCF1]
ATGAGCCACGTGGTGGACCTTGATATGGGCAGGATTCTGATCGATTTGTCGGACGACGTCATTCAACGGCTGGATAACCT
TAAACAGTTGAGAAACCAGCCGCGCGCCGAGTTATTGCGTGAGGCCATTGAGCAATATCTCGATAAACAAAGCTCCACGG
TGATTCGGGAGGCTCTTGGTTTATGGGGAAATCAGGAAGAGGATGGGCTTGAATACGAACGCAAGCTTCGTGAGGAGTGG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A806X7M6