Detailed information of TA system TA024948

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4119304..4119938
• Replicon: chromosome
• Accession: NC_014618
• Organism: Enterobacter lignolyticus SCF1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: E3GBM8
• Locus tag: ENTCL_RS23215
• Protein ID: WP_013367793.1
• Coordinates: 4119304..4119600 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: E3GBM9
• Locus tag: ENTCL_RS19135
• Protein ID: WP_013367794.1
• Coordinates: 4119621..4119938 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENTCL_RS19105	4114790..4115488	+	699	WP_125451901.1	cache domain-containing protein	-
ENTCL_RS19110	4115574..4117049	-	1476	WP_013367789.1	APC family permease	-
ENTCL_RS19115	4117372..4117767	+	396	WP_013367790.1	DUF1398 family protein	-
ENTCL_RS19120	4118141..4118395	+	255	WP_013367791.1	helix-turn-helix domain-containing protein	-
ENTCL_RS19125	4118515..4118850	+	336	WP_013367792.1	DUF1294 domain-containing protein	-
ENTCL_RS23215	4119304..4119600	-	297	WP_013367793.1	TA system toxin CbtA family protein	Toxin
ENTCL_RS19135	4119621..4119938	-	318	WP_013367794.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ENTCL_RS19140	4119956..4120177	-	222	WP_013367795.1	DUF987 domain-containing protein	-
ENTCL_RS19145	4120186..4120668	-	483	WP_013367796.1	DNA repair protein RadC	-
ENTCL_RS19150	4120677..4121135	-	459	WP_013367797.1	antirestriction protein	-
ENTCL_RS19155	4121221..4121457	-	237	WP_013367798.1	DUF905 domain-containing protein	-
ENTCL_RS23220	4121557..4122465	-	909	WP_013367799.1	hypothetical protein	-
ENTCL_RS19165	4122528..4122701	-	174	Protein_3821	DUF4339 domain-containing protein	-
ENTCL_RS19170	4122739..4123437	-	699	WP_013367801.1	DeoR family transcriptional regulator	-
ENTCL_RS19175	4123646..4124470	-	825	WP_013367802.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4118515..4155963	37448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-98)

Antitoxin

• Antitoxin CbeA (5-103)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11153.92 Da
• Isoelectric Point: 6.4537

>T24948 WP_013367793.1 NC_014618:c4119600-4119304 [[Enterobacter] lignolyticus SCF1]
MKTLPATTPQAATLCLSPVAVWQMLLTRLLEQHYGITLNDIPFSDETVIQEHIDAGITLANAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVNILRAR

Nucleotide

• Length: 297 bp

>T24948 NC_014618:c4119600-4119304 [[Enterobacter] lignolyticus SCF1]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCCGTGGCAGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGTATTACGCTAAACGATATACCGTTCAGTGATGAAACGGTTATACAGGAACATATCG
ATGCCGGAATTACTCTGGCTAATGCCGTTAACTTTTTGGTGGAGAAATATGAATTGGTTCGTATCGATCGCAAAGGATTT
AGCTGGCAAGAGCAGTCCCCATATCTTCGGGCTGTAAATATTCTGCGGGCACGATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11867.48 Da
• Isoelectric Point: 6.4776

>AT24948 WP_013367794.1 NC_014618:c4119938-4119621 [[Enterobacter] lignolyticus SCF1]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRVSITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRHAHCVTLYH
DGFTCEADTLGSYGYVYIAIYPTHS

Nucleotide

• Length: 318 bp

>AT24948 NC_014618:c4119938-4119621 [[Enterobacter] lignolyticus SCF1]
ATGAGCAACATCACATGGGGCCTGCAGCGAGATATCACGCCACGCCTAGGAGCTCGTCTGGTGCAGGAGGGTAACCGGCT
GCACTATCTGGCTGACCGGGTCAGTATCACGGGTAAGTTCAGCGAAGCAGAATGTCTTAAGCTGGATGTGGCATTCCCGC
ATTTTATCAGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
GATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGCCATTTACCCCACTCATAGTTAA

Structures

Toxin

• AlphaFold DB: E3GBM8

Antitoxin

• AlphaFold DB: E3GBM9