Detailed information of TA system TA249465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 515684..517404
• Replicon: chromosome
• Accession: NZ_CP099808
• Organism: Yersinia ruckeri strain NVI-11076

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: LNQ41_RS02375
• Protein ID: WP_038245758.1
• Coordinates: 515684..516340 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: LNQ41_RS02380
• Protein ID: WP_038245756.1
• Coordinates: 516337..517404 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNQ41_RS02355 (LNQ41_002355)	513794..514048	+	255	WP_005175264.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
LNQ41_RS02360 (LNQ41_002360)	514038..514328	+	291	WP_038245762.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LNQ41_RS02365 (LNQ41_002365)	514592..515059	+	468	WP_051847072.1	restriction endonuclease subunit S	-
LNQ41_RS02370 (LNQ41_002370)	515106..515681	+	576	WP_038245760.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
LNQ41_RS02375 (LNQ41_002375)	515684..516340	+	657	WP_038245758.1	DUF4433 domain-containing protein	Toxin
LNQ41_RS02380 (LNQ41_002380)	516337..517404	+	1068	WP_038245756.1	macro domain-containing protein	Antitoxin
LNQ41_RS02385 (LNQ41_002385)	517425..517691	+	267	Protein_462	hypothetical protein	-
LNQ41_RS02390 (LNQ41_002390)	517710..518108	-	399	Protein_463	integrase core domain-containing protein	-
LNQ41_RS02395 (LNQ41_002395)	518107..518403	+	297	Protein_464	integrase core domain-containing protein	-
LNQ41_RS02400 (LNQ41_002400)	518432..518537	-	106	Protein_465	transposase	-
LNQ41_RS02405 (LNQ41_002405)	518551..518679	-	129	Protein_466	helix-turn-helix domain-containing protein	-
LNQ41_RS02410 (LNQ41_002410)	518829..519059	+	231	Protein_467	DUF4942 domain-containing protein	-
LNQ41_RS02415 (LNQ41_002415)	519134..519529	+	396	WP_038245317.1	DUF6088 family protein	-
LNQ41_RS02420 (LNQ41_002420)	519522..520471	+	950	Protein_469	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
LNQ41_RS02425 (LNQ41_002425)	520842..520967	+	126	Protein_470	antirestriction protein	-
LNQ41_RS02430 (LNQ41_002430)	520993..521166	+	174	Protein_471	type IV toxin-antitoxin system YeeU family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	513794..523663	9869
	-	inside	IScluster/Tn	-	-	512408..518403	5995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25244.74 Da
• Isoelectric Point: 6.6007

>T249465 WP_038245758.1 NZ_CP099808:515684-516340 [Yersinia ruckeri]
VAYDYSTSLNPEKALIWRIVHRDNIPWILDNGLHCGNSPVQAEDWINIGNPELIDKRAGHLVPTGMRGTLHDYVPFYFTP
FSPMLMNIRSGRGGIKQRPNEEIVILVSSLRNMVAQNVSFVFTDSHAYYNLTNYFTELDSLNQIDWPILQARDFRRDQDD
LAKFERYQAEALIWKHCPIALLGGMICYNDGVRLQLEQWLSQRNLSMPVHARAGWYFS

Nucleotide

• Length: 657 bp

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40846.27 Da
• Isoelectric Point: 6.6976

>AT249465 WP_038245756.1 NZ_CP099808:516337-517404 [Yersinia ruckeri]
MITFTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPVNMKAYALACKHKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWVEDGLQDLRRFLIKEKVQSIAIPPLGAGNGGLNWSDVRMRIESALGDLQDTEILIYEPTEKYQNVAKST
GVKKLTPARALIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDTQPLDVIWFNDQEKEHINLWLNNEAKEWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGKCQPTLDSVKEG
LHQWPAGERWASRKMKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp