249446

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 1856075..1857583 Replicon chromosome
Accession NZ_CP099798
Organism Pseudomonas aeruginosa strain PAO1-L

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag NHZ68_RS08645 Protein ID WP_003119682.1
Coordinates 1856075..1856662 (+) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9HYX2
Locus tag NHZ68_RS08650 Protein ID WP_003115566.1
Coordinates 1856729..1857583 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHZ68_RS08640 (NHZ68_08640) 1852330..1855809 - 3480 WP_003114080.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -
NHZ68_RS08645 (NHZ68_08645) 1856075..1856662 + 588 WP_003119682.1 GNAT family protein Toxin
NHZ68_RS08650 (NHZ68_08650) 1856729..1857583 + 855 WP_003115566.1 AraC family transcriptional regulator Antitoxin
NHZ68_RS08655 (NHZ68_08655) 1857680..1859845 + 2166 WP_003115567.1 TonB-dependent receptor family protein -
NHZ68_RS08660 (NHZ68_08660) 1860020..1861894 + 1875 WP_003091605.1 MFS transporter -
NHZ68_RS08665 (NHZ68_08665) 1861955..1862164 - 210 WP_003115568.1 cold-shock protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T249446 WP_003119682.1 NZ_CP099798:1856075-1856662 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31933.23 Da        Isoelectric Point: 7.3276

>AT249446 WP_003115566.1 NZ_CP099798:1856729-1857583 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Download         Length: 855 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB Q9HYX2

References