Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4576818..4577315 Replicon chromosome
Accession NZ_CP099754
Organism Escherichia coli strain 806883-9-2019

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NH020_RS22095 Protein ID WP_001173343.1
Coordinates 4576818..4576991 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NH020_RS22100 Protein ID WP_000398619.1
Coordinates 4577019..4577315 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH020_RS22085 (4573488) 4573488..4574945 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
NH020_RS22090 (4575182) 4575182..4576699 + 1518 WP_022646456.1 lysine--tRNA ligase -
NH020_RS22095 (4576818) 4576818..4576991 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NH020_RS22100 (4577019) 4577019..4577315 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NH020_RS22105 (4577544) 4577544..4577816 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NH020_RS22110 (4577828) 4577828..4578058 - 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
NH020_RS22115 (4578239) 4578239..4579870 + 1632 WP_022646455.1 sensor histidine kinase -
NH020_RS22120 (4579867) 4579867..4580586 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4552906..4577315 24409


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T249432 WP_001173343.1 NZ_CP099754:c4576991-4576818 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT249432 WP_000398619.1 NZ_CP099754:c4577315-4577019 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References