Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4357005..4357581 Replicon chromosome
Accession NZ_CP099754
Organism Escherichia coli strain 806883-9-2019

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NH020_RS20970 Protein ID WP_001297643.1
Coordinates 4357294..4357581 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NH020_RS20965 Protein ID WP_000063156.1
Coordinates 4357005..4357307 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH020_RS20950 (4353601) 4353601..4355751 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NH020_RS20955 (4355801) 4355801..4356004 + 204 WP_000467859.1 YbdD/YjiX family protein -
NH020_RS20960 (4356015) 4356015..4356971 + 957 WP_001297640.1 GTPase -
NH020_RS20965 (4357005) 4357005..4357307 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NH020_RS20970 (4357294) 4357294..4357581 - 288 WP_001297643.1 BrnT family toxin Toxin
NH020_RS20980 (4358534) 4358534..4358629 + 96 Protein_4112 dienelactone hydrolase family protein -
NH020_RS20985 (4358775) 4358775..4360007 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
NH020_RS20990 (4360048) 4360048..4361328 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4357780..4358073 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T249429 WP_001297643.1 NZ_CP099754:c4357581-4357294 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT249429 WP_000063156.1 NZ_CP099754:c4357307-4357005 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References