Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3046791..3047575 Replicon chromosome
Accession NZ_CP099754
Organism Escherichia coli strain 806883-9-2019

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag NH020_RS14600 Protein ID WP_000613626.1
Coordinates 3047081..3047575 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag NH020_RS14595 Protein ID WP_024946541.1
Coordinates 3046791..3047084 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH020_RS14585 (3041952) 3041952..3042911 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
NH020_RS14590 (3043484) 3043484..3046657 + 3174 WP_022645526.1 ribonuclease E -
NH020_RS14595 (3046791) 3046791..3047084 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
NH020_RS14600 (3047081) 3047081..3047575 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
NH020_RS14605 (3047670) 3047670..3048623 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
NH020_RS14610 (3048635) 3048635..3050278 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
NH020_RS14615 (3050344) 3050344..3051285 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
NH020_RS14620 (3051285) 3051285..3052382 - 1098 WP_022645522.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T249423 WP_000613626.1 NZ_CP099754:3047081-3047575 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT249423 WP_024946541.1 NZ_CP099754:3046791-3047084 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References