Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3857310..3857964 Replicon chromosome
Accession NZ_CP099752
Organism Escherichia coli strain 806883-11-2019

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NHF43_RS18570 Protein ID WP_000244777.1
Coordinates 3857557..3857964 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NHF43_RS18565 Protein ID WP_000354046.1
Coordinates 3857310..3857576 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF43_RS18540 (3852479) 3852479..3853222 + 744 WP_000951964.1 SDR family oxidoreductase -
NHF43_RS18545 (3853279) 3853279..3854712 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
NHF43_RS18550 (3854757) 3854757..3855068 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
NHF43_RS18555 (3855232) 3855232..3855891 + 660 WP_000250274.1 hemolysin III family protein -
NHF43_RS18560 (3856087) 3856087..3857067 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NHF43_RS18565 (3857310) 3857310..3857576 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NHF43_RS18570 (3857557) 3857557..3857964 + 408 WP_000244777.1 protein YgfX Toxin
NHF43_RS18575 (3858004) 3858004..3858525 - 522 WP_001055874.1 flavodoxin FldB -
NHF43_RS18580 (3858637) 3858637..3859533 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NHF43_RS18585 (3859558) 3859558..3860268 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NHF43_RS18590 (3860274) 3860274..3862007 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T249409 WP_000244777.1 NZ_CP099752:3857557-3857964 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT249409 WP_000354046.1 NZ_CP099752:3857310-3857576 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References