Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2504180..2504677 Replicon chromosome
Accession NZ_CP099752
Organism Escherichia coli strain 806883-11-2019

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NHF43_RS12135 Protein ID WP_001173343.1
Coordinates 2504180..2504353 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NHF43_RS12140 Protein ID WP_000398619.1
Coordinates 2504381..2504677 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF43_RS12125 (2500850) 2500850..2502307 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
NHF43_RS12130 (2502544) 2502544..2504061 + 1518 WP_001295074.1 lysine--tRNA ligase -
NHF43_RS12135 (2504180) 2504180..2504353 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NHF43_RS12140 (2504381) 2504381..2504677 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NHF43_RS12145 (2504904) 2504904..2505176 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NHF43_RS12150 (2505188) 2505188..2505418 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
NHF43_RS12155 (2505599) 2505599..2507230 + 1632 WP_001216461.1 sensor histidine kinase -
NHF43_RS12160 (2507227) 2507227..2507946 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T249405 WP_001173343.1 NZ_CP099752:c2504353-2504180 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT249405 WP_000398619.1 NZ_CP099752:c2504677-2504381 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References