249403

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1749279..1750116 Replicon chromosome
Accession NZ_CP099752
Organism Escherichia coli strain 806883-11-2019

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NHF43_RS08515 Protein ID WP_000227784.1
Coordinates 1749574..1750116 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NHF43_RS08510 Protein ID WP_001297137.1
Coordinates 1749279..1749590 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF43_RS08485 (1744299) 1744299..1745246 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
NHF43_RS08490 (1745268) 1745268..1747259 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NHF43_RS08495 (1747249) 1747249..1747863 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NHF43_RS08500 (1747863) 1747863..1748192 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NHF43_RS08505 (1748204) 1748204..1749094 + 891 WP_000971336.1 heme o synthase -
NHF43_RS08510 (1749279) 1749279..1749590 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NHF43_RS08515 (1749574) 1749574..1750116 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NHF43_RS08520 (1750172) 1750172..1751107 - 936 WP_001297127.1 tetratricopeptide repeat protein -
NHF43_RS08525 (1751515) 1751515..1752879 + 1365 WP_001000978.1 MFS transporter -
NHF43_RS08530 (1753007) 1753007..1753498 - 492 WP_001138904.1 nucleotide binding protein YajQ -
NHF43_RS08535 (1753666) 1753666..1754577 + 912 WP_000705849.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T249403 WP_000227784.1 NZ_CP099752:1749574-1750116 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT249403 WP_001297137.1 NZ_CP099752:1749279-1749590 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References