Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5273015..5273640 Replicon chromosome
Accession NZ_CP099748
Organism Klebsiella pneumoniae strain 806883-10-2019

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NHF42_RS25475 Protein ID WP_002882817.1
Coordinates 5273015..5273398 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NHF42_RS25480 Protein ID WP_004150355.1
Coordinates 5273398..5273640 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF42_RS25460 (NHF42_25445) 5270381..5271283 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NHF42_RS25465 (NHF42_25450) 5271280..5271915 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NHF42_RS25470 (NHF42_25455) 5271912..5272841 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NHF42_RS25475 (NHF42_25460) 5273015..5273398 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NHF42_RS25480 (NHF42_25465) 5273398..5273640 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NHF42_RS25485 (NHF42_25470) 5273845..5274762 + 918 WP_004895173.1 alpha/beta hydrolase -
NHF42_RS25490 (NHF42_25475) 5274776..5275717 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NHF42_RS25495 (NHF42_25480) 5275762..5276199 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NHF42_RS25500 (NHF42_25485) 5276196..5277056 - 861 WP_004146232.1 virulence factor BrkB family protein -
NHF42_RS25505 (NHF42_25490) 5277050..5277649 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T249389 WP_002882817.1 NZ_CP099748:c5273398-5273015 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT249389 WP_004150355.1 NZ_CP099748:c5273640-5273398 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References