Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5234019..5234644 Replicon chromosome
Accession NZ_CP099743
Organism Klebsiella pneumoniae strain 503666-2-2019

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NHF44_RS25305 Protein ID WP_002882817.1
Coordinates 5234019..5234402 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NHF44_RS25310 Protein ID WP_004150355.1
Coordinates 5234402..5234644 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF44_RS25290 (NHF44_25275) 5231385..5232287 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NHF44_RS25295 (NHF44_25280) 5232284..5232919 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NHF44_RS25300 (NHF44_25285) 5232916..5233845 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NHF44_RS25305 (NHF44_25290) 5234019..5234402 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NHF44_RS25310 (NHF44_25295) 5234402..5234644 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NHF44_RS25315 (NHF44_25300) 5234849..5235766 + 918 WP_004895173.1 alpha/beta hydrolase -
NHF44_RS25320 (NHF44_25305) 5235780..5236721 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NHF44_RS25325 (NHF44_25310) 5236766..5237203 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NHF44_RS25330 (NHF44_25315) 5237200..5238060 - 861 WP_004146232.1 virulence factor BrkB family protein -
NHF44_RS25335 (NHF44_25320) 5238054..5238653 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T249370 WP_002882817.1 NZ_CP099743:c5234402-5234019 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT249370 WP_004150355.1 NZ_CP099743:c5234644-5234402 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References